| Species |
Human |
| Protein Construction |
Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer [Phe20-Pro1107(ITGAX) acidic tail & Gln23-Asn700(ITGB2) basic tail]
Accession # P20702-1(ITGAX)&P05107-1(ITGB2) |
His |
| N-term |
C-term |
|
| Purity |
> 95% as determined by BisTris PAGE
> 95% as determined by HPLC |
| Endotoxin Level |
Less than 1EU per μg by the LAL method. |
| Biological Activity |
Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha X beta 2 (ITGAX&ITGB2) Heterodimer, His, Human at 2μg/ml (100μl/well) on the plate can bind AntiITGB2 Antibody, hFc Tag. Test result was comparable to standard batch. |
| Expression System |
HEK293 |
| Theoretical Molecular Weight |
125.50 kDa (ITGAX) and 79.50 kDa (ITGB2) |
| Apparent Molecular Weight |
Due to glycosylation, the protein migrates to 130-135 kDa (ITGAX) and 85-95 kDa (ITGB2) based on Bis-Tris PAGE result. |
| Formulation |
Lyophilized from 0.22μm filtered solution in PBS (pH 7.4). |
| Reconstitution |
Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water. |
| Storage & Stability |
Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles. |
| Target Background |
The integrin alpha X beta 2 (CD11c/CD18, p150,95) binds ligands through the I domain of the alpha X subunit. Ligands include the complement factor fragment iC3b, a key component in the innate immune defense, which, together with the expression of alpha X beta 2 on dendritic cells and on other leukocytes, suggests a role in the immune response. |
| Synonyms |
αXβ2; CD11C; SLEB6; CD18; ITGAX&ITGB2; ITGA; ITGB2 |
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
