目录产品 » Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human
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Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human

Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC­SIGN[2].
￥4100
Z05527-100

产品简介
Species Human
Protein Construction
Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer [Phe17-Asn1104 (ITGAM) acidic tail and Gln23-Asn700(ITGB2) basic tail]
Accession # P11215-1(ITGAM)&P05107-1(ITGB2)		 His
N-term C-term
Purity > 95% as determined by Bis-Tris PAGE
Endotoxin Level Less than 1 EU per μg by the LAL method.
Biological Activity Measured by its binding ability in a functional ELISA. Immobilized Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human at 2μg/ml (100 μl/well) on the plate can bind Anti­ITGB2 Antibody, hFc Tag. Test result was comparable to standard batch.
Expression System HEK293
Theoretical Molecular Weight 126.10 kDa (ITGAM) and 79.50 kDa (ITGB2)
Apparent Molecular Weight Due to glycosylation, the protein migrates to 140-160 kDa (ITGAM) and 90-100 kDa (ITGB2) based on Bis-Tris PAGE result.
Formulation Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).
Reconstitution Centrifuge the tube before opening. Reconstituting to a concentration more than 100 μg/ml is recommended. Dissolve the lyophilized protein in distilled water.
Storage & Stability Upon receiving, the product remains stable up to 6 months at -20 °C or below. Upon reconstitution, the product should be stable for 3 months at -80 °C. Avoid repeated freeze-thaw cycles.
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图例
Integrin Alpha M Beta 2 (ITGAM&ITGB2) Heterodimer, His, Human

Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human, His Tag at 2 μg/ml (100 μl/well) on the plate. Dose response curve for Anti-ITGB2 Antibody, hFc Tag with the EC50 of 13.2 ng/ml determined by ELISA (QC Test). »

Integrin Alpha M Beta 2 (ITGAM&ITGB2) Heterodimer, His, Human

Integrin alpha M beta 2 (ITGAM&ITGB2) Heterodimer, His, Human on Bis-Tris PAGE under reduced condition. The purity is greater than 95%. »

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技术背景
Target Background Integrin αMβ2, also called Mac-1 or complement receptor-3 (CR3), is a heterodimer expressed on monocytes. It functions as both an adhesion molecule mediating the diapedesis of leukocytes across the endothelium and a receptor for the iC3b fragment of complement responsible for phagocytic/degranulation responses to microorganisms[1]. Active αMβ2 binds iC3b, coagulation proteins fibrinogen, plasminogen and factor X, extracellular matrix (ECM) proteins fibronectin, laminin and collagen, and cell surface ICAMs, myelin basic protein and DC­SIGN[2].
Synonyms αMβ2; ITGAM&ITGB2; ITGAM; ITGB2
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For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.