THE™ c-Myc Tag Antibody, mAb, Mouse
| Synonyms | Mouse Anti-c-Myc-tag mAb; |
| Description | The c-Myc protein is a transcription factor, encoded by the c-Myc gene on human chromosome 8q24. c-Myc is commonly activated in a variety of tumor cells and plays an important role in cellular proliferation, differentiation, apoptosis, and cell cycle progression. A synthetic peptide corresponding to residues 410-419 of the human p62 c-myc protein conjugated to KLH is used as immunogen. c-Myc-tag provides a method of localizing gene products in a variety of cell types, to study the topology of proteins and protein complexes and of identifying associated proteins. This product is suitable for detecting the expression level of c-Myc fusion proteins or c-Myc by various immunoassays. |
| Host Species | Mouse |
| Conjugation | Unconjugated |
| Immunogen | A synthetic peptide EQKLISEEDL coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells |
| Purification | Protein A affinity column |
| Key Features |
| Subclass | IgG2a, k |
| Clone ID | 2G8D5 |
| Concentration | 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution | Reconstitute the lyophilized antibody with deionized water (or equivalent) to the final concentration of 0.5 mg/ml. |
| Specificity | THETM c-Myc Tag Antibody, mAb, Mouse recognizes C-terminal, N-terminal, and internal c-Myc tagged fusion proteins expressed in prokaryotic or eukaryotic cells. It also recognizes c-Myc protein in human cancers or cell lines such as Hela cell lysates. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05 µg/ml-0.2 µg/ml Western blot: 0.05 µg/ml-0.5 µg/ml Western blot using ONE-HOUR WesternTM Kit: For quick results, GenScript ONE-HOUR WesternTM Kit L00205C is recommended. 0.9 µg of this antibody is mixed with diluted WB solution (dilute 1.5 ml WB with 1.5 ml of PBST). Immunoprecipitation (IP): 1 µg/ml Immunofluorescent staining: 1 µg/ml Other applications: user-optimized |
TK Hyun, et al. De-novo RNA sequencing and metabolite profiling to identify genes involved in anthocyanin biosynthesis in Korean black raspberry (Rubus coreanus Miquel). PLoS One. 2014Feb.;9(2):
Daniel K. Stringer and Robert C. Piper..,et al. A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination.J Cell Biol.2011Jan;192(2):229 - 242
Floria Faradji.,et al. Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation.Cell Cycle.2011Mar;10(5):805-18
Steven M. Markus.,et al. Quantitative analysis of Pac1/LIS1â€mediated dynein targeting: Implications for regulation of dynein activity in budding yeast.Cytoskeleton (Hoboken).2011Mar;68(3):157-74
Natalya L. Teterina.,et al. Analysis of Poliovirus Protein 3A Interactions with Viral and Cellular Proteins in Infected Cells.J Virol.2011May;85(9):4284 - 4296
Zhu M.,et al. Investigation of the influence of Arg555Trp and Thr538Pro TGFBI mutations on C-terminal cleavage and cell endoplasmic reticulum stress.Mol Vis.2012Jan;18:1156-64
Campos SK.,et al. Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration.J Virol.2012Apr;86(8):4169-81
HJ Koo.,et al. Suites of Terpene Synthases Explain Differential Terpenoid Production in Ginger and Turmeric Tissues.PLoS One.2012Dec;7(12):e51481
Ridley CM.,et al. β-Glucosidase 2 (GBA2) Activity and Imino Sugar Pharmacology.J Biol Chem.2013Sep;288(36):26052-66
Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;
请点击此处查找更多相关文献
Daniel K. Stringer and Robert C. Piper..,et al. A single ubiquitin is sufficient for cargo protein entry into MVBs in the absence of ESCRT ubiquitination.J Cell Biol.2011Jan;192(2):229 - 242
Floria Faradji.,et al. Drosophila melanogaster Cyclin G coordinates cell growth and cell proliferation.Cell Cycle.2011Mar;10(5):805-18
Steven M. Markus.,et al. Quantitative analysis of Pac1/LIS1â€mediated dynein targeting: Implications for regulation of dynein activity in budding yeast.Cytoskeleton (Hoboken).2011Mar;68(3):157-74
Natalya L. Teterina.,et al. Analysis of Poliovirus Protein 3A Interactions with Viral and Cellular Proteins in Infected Cells.J Virol.2011May;85(9):4284 - 4296
Zhu M.,et al. Investigation of the influence of Arg555Trp and Thr538Pro TGFBI mutations on C-terminal cleavage and cell endoplasmic reticulum stress.Mol Vis.2012Jan;18:1156-64
Campos SK.,et al. Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration.J Virol.2012Apr;86(8):4169-81
HJ Koo.,et al. Suites of Terpene Synthases Explain Differential Terpenoid Production in Ginger and Turmeric Tissues.PLoS One.2012Dec;7(12):e51481
Ridley CM.,et al. β-Glucosidase 2 (GBA2) Activity and Imino Sugar Pharmacology.J Biol Chem.2013Sep;288(36):26052-66
Pawlisz AS.,et al. Three-dimensional regulation of radial glial functions by Lis1-Nde1 and dystrophin glycoprotein complexes.PLoS Biol.2011Otc;
请点击此处查找更多相关文献
| 产品编号: A00704 | ||
|---|---|---|
规格 |
价格 |
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| 100 μg | ¥1500 | |
