A Guide to CRISPR /Cas9 Delivery: How to Maximize Your Editing Efficiency CRISPR gRNA Plasmids and Vectors crRNA/Cas9 Protein Reagents Cell Line Genome Editing All-in-one gRNA/Cas9 or dual vectors available
The current applications for CRISPR/Cas9 in genome editing.
针对目标序列设计的gRNA与Cas9结合,并将Cas9引导到目标序列,目标序列的PAM 序列上游 3-4 个碱基处,Cas9切割产生双链断裂(DSB)。
针对目标序列设计的gRNA与Cas9结合,并将Cas9引导到目标序列,目标序列的PAM序列上游3-4个碱基处,Cas9切割产生双链断裂(DSB)。
One of the DNA repair mechanisms triggered by CRISPR/Cas9 is the homology directed repair (HDR) process, in which DNA templates can be inserted into double-stranded breaks created by Cas9 via homologous
Can CRISPR/Cas9 off-target genomic editing be avoided?
Free Download From CRISPR/Cas9 fundamental mechanisms to the tools and methods enabling today’s non-viral CRISPR/Cas9 mediated insertions of large synthetic gene sequences.
CRISPR/Cas9 has impacted nearly every area of genetic research.
Finding suitable sequences for Cas9 targeting.
Design high specificity CRISPR/Cas9 gRNAs: principles and tools The CRISPR/Cas9 system has recently emerged as the most powerful gene editing method to study gene function.