利用RNP复合物(Cas9和sgRNA形成的核糖核蛋白)替代外源载体,通过脂质体、电转染、显微注射或细胞传膜肽等途径直接转入宿主细胞,具有快捷安全、脱靶效应更低、编辑效率更高等优势,逐渐成为CRISPR/Cas9技术更高效的实现途径。
Increasing CRISPR/Cas9 Editing Specificity CRISPR Cas9 based gene knockout relies on the specificity of both the sgRNA and the Cas9 protein.
GenCRISPR™/Cas9基因编辑相关产品 概览 联系我们 导航 概览 联系我们 GenCRISPR™/Cas9基因编辑系统工具 CRISPR/Cas9是一种强大的基因编辑工具。
In addition to live DNA imaging, the CRISPR/Cas9 system can be used for live RNA imaging as well.
Check Out These Recent Papers That Used CRISPR/Cas9 in Several Different Species Repurposing CRISPR/Cas9 for in situ functional assays.
传统的CRISPR/Cas9基因编辑方法借助于Cas9和gRNA表达质粒,转染宿主细胞后在宿主细胞内通过转录生成gRNA并生成Cas9蛋白,而后发挥基因编辑效应。
Double strand breaks are induced by Cas9 (e.g., spCas9), which are repaired by the endogenous cell repair pathways (i.e.
Editing Solutions All solutions you need for a successful CRISPR gene editing project Free Download Related Products Cas9/Cas12a/Cas13a Nucleases Off-the-shelf Cas9, Cas12a and Cas13 nuclease products
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