HEK293/TLR2/CD14/NFκB-Luc
HEK293 cells overexpressing TLR2, CD14 protein and luciferase driven by NF-κB RE to screen the leads targeting TLR2
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| RD00974 | |
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| Product Description | HEK293 cells overexpressing TLR2, CD14 protein and luciferase driven by NF-κB RE to screen the leads targeting TLR2 |
| Culture Properties | Adherent |
| Product Type | Cell line |
| Stability | Stable through more than 15 passages with no significant changes in assay performance or expression profile. |
| Size | 2 vials of frozen cells (>3X106 per vial in 1 ml) |
| Storage | Store cells in liquid nitrogen immediately upon receipt. Thaw and recover cells within one year from the date received. |
| Culture Medium | DMEM, 10% FBS, 1 μg/mL Puromycin, 200 μg/mL Hygromycin B, 600 μg/mL G418 |
| Complete Growth Medium | DMEM with 10% FBS |
| Freeze Medium-DATA | 90% complete growth medium, 10% (V/V) DMSO Or OptiVitro® Cell Cryopreservation Medium |
Flow cytometric analysis of HEK293/TLR2/CD14 cells stained with anti-human CD282 (TLR2) antibody (BioLegend, Cat. No.: 392304) after cell digestion using Accutase Cell Dissociation Reagent. »
Flow cytometric analysis of HEK293/TLR2/CD14 cells stained with anti-human CD14 antibody (BioLegend, Cat. No.: 367104) after cell digestion using Accutase Cell Dissociation Reagent. »
Pam3CSK4, acting as an agonist, was incubated with HEK293/TLR2/CD14/NF-κB cells at 37°C, 5% CO2 for 18 hours. Subsequently, luciferase substrate was added, and the luminescence was measured using the PHERAstar FSX. Data were analyzed by GraphPad Prism® software. »
For research use only. Not intended for human or animal clinical trials, therapeutic or diagnostic use.
