|Synonyms||THETM V5 Tag Antibody [iFluor 647], mAb, Mouse; V5 Tag Antibody [iFluor 647], mAb, Mouse; 647 conjugated V5 antibody; 647 conjugated V5 monoclonal antibody|
|Description||The V5 tag is a polypeptide of GKPIPNPLLGLDST amino acid sequence that can be added to a target protein using recombinant DNA technology. It can be fused to the N-terminus, C-terminus or inserted in an internal region of the protein to facilitate detection and purification. An anti-V5 tag antibody is a useful tool for the analysis of V5-tagged proteins.
THETM V5 Tag Antibody [iFluor 647], mAb, Mouse is THETM V5 Tag Antibody (A01724) conjugated with iFluor 647 under optimal conditions with an F/P ratio of 3-6. It is suitable for detecting the expression level of V5-tagged proteins. iFluor 647 is a bright and photostable fluorescent dye. It is an excellent alternative to Alexa Fluor 647.
|Immunogen||A synthetic peptide GKPIPNPLLGLDST coupled to KLH|
|Purification||Protein A affinity column|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 10 mg/ml BSA and 0.02% sodium azide.|
|Reconstitution||Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.|
|Specificity||THETM V5 Tag Antibody [iFluor 647], mAb, Mouse recognizes N-terminal, internal and C-terminal V5-tagged proteins.
Fluorescent Dye Characterization
|Storage||The lyophilized product remains stable up to 2 year at -20°C from date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8°C or for up to 12 months at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescent staining: 1-4 µg/ml
Flow cytometry: 1-4 µg/ml
Other applications: user-optimized