利用RNP复合物(Cas9和sgRNA形成的核糖核蛋白)替代外源载体,通过脂质体、电转染、显微注射或细胞传膜肽等途径直接转入宿主细胞,具有快捷安全、脱靶效应更低、编辑效率更高等优势,逐渐成为CRISPR/Cas9技术更高效的实现途径。
GenCRISPR™/Cas9基因编辑相关产品 概览 联系我们 导航 概览 联系我们 GenCRISPR™/Cas9基因编辑系统工具 CRISPR/Cas9是一种强大的基因编辑工具。
Check Out These Recent Papers That Used CRISPR/Cas9 in Several Different Species Repurposing CRISPR/Cas9 for in situ functional assays.
传统的CRISPR/Cas9基因编辑方法借助于Cas9和gRNA表达质粒,转染宿主细胞后在宿主细胞内通过转录生成gRNA并生成Cas9蛋白,而后发挥基因编辑效应。
Increasing CRISPR/Cas9 Editing Specificity CRISPR Cas9 based gene knockout relies on the specificity of both the sgRNA and the Cas9 protein.
In addition to live DNA imaging, the CRISPR/Cas9 system can be used for live RNA imaging as well.
Double strand breaks are induced by Cas9 (e.g., spCas9), which are repaired by the endogenous cell repair pathways (i.e.
Editing Solutions All solutions you need for a successful CRISPR gene editing project Free Download Related Products Cas9/Cas12a/Cas13a Nucleases Off-the-shelf Cas9, Cas12a and Cas13 nuclease products
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