Naeimi Kararoudi et al. present an
efficient method using CRISPR and AAV
for site-directed gene knockin into human
primary NK cells and show proof of
concept by generating CD33-specific
CAR-NK cells and demonstrating efficacy
against AML. This approach has wide
potential for therapeutic applications and
the study of NK cell biology.
Naeimi Kararoudi et al. present an
efficient method using CRISPR and AAV
for site-directed gene knockin into human
primary NK cells and show proof of
concept by generating CD33-specific
CAR-NK cells and demonstrating efficacy
against AML. This approach has wide
potential for therapeutic applications and
the study of NK cell biology.
