| ￥2500 | |
| A02014 | |
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| Specificity |
GenScript’s MonoRab™ Rabbit Anti-Camelid VHH Cocktail is specific for
Camelid VHH antibody (Llama, Alpaca, and Camel). It has no cross-reactivity
with mouse, rat, rabbit, goat or human immunoglobulins. |
| Host Species | Rabbit |
| Immunogen | Llama VHH antibody |
| Conjugate | Unconjugated |
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Working concentrations for specific applications should be determined by
the investigators. The appropriate concentrations may be affected by
primary/secondary antibody affinity, antigen concentration, the sensitivity of
the method of detection, temperature, the length of the incubations, and other
factors. The suitability of this antibody for applications other than those
listed below has not been determined. The following concentration ranges are
recommended starting points for this product. Optimal working dilutions must be
determined by the end user. |
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| Form | Liquid |
| Storage Buffer | Supplied in PBS (pH 7.4) with 0.02% sodium azide and 50% glycerol. |
| Concentration | 0.5 mg/mL |
| Storage Instructions | Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed. |
| Purification | Protein A affinity column |
| Isotype | Rabbit IgG |
| Clonality | Cocktail |
| Note | GenScript can customize this product per customer's request including product size, buffer components, etc. |
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Figure 1. The specificity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) to VHH-transduced CAR-T cells was tested by immunocytochemical/immunofluorescence analysis of VHH-transduced CAR-T cells infiltrated NCG mouse spleen.
VHH was labelled with MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) at 0.5 µg/mL, and anti-rabbit IgG (H+L) (Alexa Fluor 488) was used as secondary antibody at 1/500 concentration (4 μg/mL; green).
APC hCD45 monoclonal antibody was used to define all infused T cells at 1/50 concentration (0.5 μg/mL; red).
Confocal images showed cytoplasmic staining in infiltrated VHH-transduced CAR-T cells in the NCG mouse spleen.
The untransduced infiltrated T cells showed a negative signal with MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014).
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Figure 2. The specificity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) to VHH-transduced CAR-T cells was tested by Immunohistochemical analysis of VHH-transduced CAR-T cells infiltrated liver tissue of PDX mouse. VHH was labelled with MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) at 1 μg/mL, followed by incubation with biotinylated anti-rabbit IgG (H+L) (2.5 μg/mL). The final signal was amplified by ABC (Avidin-Biotin Complex) Kit.
Positive staining was shown in the membrane or cytoplasm of VHH-transduced CAR-T cells infiltrated into liver tissue. Counterstained with hematoxylin.
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Figure 3. The affinity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014) for different VHHs was analyzed by Western blotting using MonoRab™ Rabbit Anti-Camelid VHH Cocktail (GenScript, A02014, 0.5 µg/mL). The signal was developed with Goat Anti-Rabbit IgG Antibody (H&L) [HRP], pAb (Genscript, A00098, 1:5,000).
Lanes 1-3: 100 ng, 50 ng, 25 ng VHH #1
Lanes 4-6: 100 ng, 50 ng, 25 ng VHH #2
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Figure 4. The specificity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (A02014) to camelid IgG1, IgG2, and IgG3 was tested using ELISA. The results show that MonoRab™ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to camelid heavy chain antibodies (IgG2 & 3), but not to the camelid conventional antibody (IgG1). »
Figure 5. The specificity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (A02014) to non-camelid IgG was tested using ELISA. The results show that MonoRab™ Rabbit Anti-Camelid VHH Cocktail (A02014) is specific to camelid IgG and has no cross-reactivity with mouse, rat, rabbit, goat and human immunoglobulins. »
Figure 6. Standard curve for quantitative detection of VHH by sandwich ELISA. The VHH Sandwich ELISA assay is developed by using MonoRab™ Rabbit Anti-Camelid VHH Antibody, mAb (GenScript, A01860) and MonoRab™ Rabbit Anti-Camelid VHH Cocktail [HRP] (GenScript, A02016) as the capture and detection antibodies, respectively. In this ELISA assay, the detection antibody was labeled with horseradish peroxidase (HRP). However, of an unconjugated detection antibody is desired, MonoRab™ Rabbit Anti-Camelid VHH Cocktail (A02014) can be used for this assay.
Note: GenScript can provide customized conjugation services for this product as per customer's request.
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Figure 7. The affinity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (Cat.no A02014) with 15 random VHHs was measured by Biacore. The cocktail antibody shows comprehensive binding activity and high affinity with all of the 15 VHHs. »
Figure 7. The affinity of MonoRab™ Rabbit Anti-Camelid VHH Cocktail (Cat.no A02014) with 15 random VHHs was measured by Biacore. The cocktail antibody shows comprehensive binding activity and high affinity with all of the 15 VHHs. »
| Target Background |
A camelid single-domain antibody (sdAb, called Nanobody by Ablynx, the
developer) is a peptide chain about 110 amino acids long, comprising one
variable domain of a heavy-chain antibody, these are called VHH fragments. Like
a whole antibody, it is able to bind selectively to a specific antigen.
Single-domain antibodies allow a broad range of applications in biotechnical as
well as therapeutic fields due to their small size, simple production and high
affinity. |
| Synonyms |
Rabbit anti-camelid VHH, single-domain antibody monoclonal antibody
cocktail (Min X Ms, Rt, Rb, Gt, Hu). |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
