目录产品 » 抗体产品 » 一抗 » 内参及对照抗体 » THE™ beta Actin Antibody, mAb, Mouse

THE™ beta Actin Antibody, mAb, Mouse

THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.
¥1500
A00702

Specificity THE™ beta Actin Antibody, mAb, Mouse reacts with mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig.It has not yet been tested in other species.
Host Species Mouse
Immunogen A synthetic peptide DDDIAALVVDNGSG coupled to KLH
Species Reactivity Mouse, rabbit, chicken, human, hamster, cow, goat, fish, and pig
Conjugate Unconjugated
返回

Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product.
Application Recommended Usage
ELISA 0.1-1.0 µg/ml
Western Blot 0.1-1.0 µg/ml
返回

Form Lyophilized
Storage Buffer Lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide.Note: 100 µg, 200µg (GenScript, A00702), lyophilized with PBS, pH 7.4, containing 0.02% sodium azide, without BSA
Reconstitution Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml.
Storage Instructions The lyophilized product remains stable for up to 1 year at -20 °C from the date of receipt. Upon reconstitution, it can be stored for 2-3 weeks at 2-8 °C or for up to 12 months at -20 °C or below. Avoid repeated freezing and thawing cycles.
Purification Protein A or G affinity column
Isotype Mouse IgG2a,κ
Clonality Monoclonal
Clone ID 2D1D10
返回

THE™ Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A, clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500) »

THE™ Beta Actin Antibody, MAb, Mouse

Primary antibody:
A. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)
B. 0.1 μg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (Company A,clone 8226)
Secondary antibody:
Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, 1:10,000, A00160)
The signal was developed with LumiSensorTM HRP Substrate Kit (GenScript, L00221V500) »

THE™ Beta Actin Antibody, MAb, Mouse

Lane 1: 20 µg Hela cell lysate
Lane 2: 20 µg sp2/0 cell lysate
Lane 3: 20 µg goat muscle lysate
Lane 4: 20 µg rabbit muscle lysate
Lane 5: 20 µg chicken muscle lysate
Lane 6: 20 µg CHO cell lysate
Lane 7: 20 µg fish muscle lysate

Primary antibody: 1 µg/ml THETM Anti-β-actin Monoclonal Antibody (Mouse) (GenScript, A00702)

Secondary antibody: Goat Anti-Mouse IgG (H&L) [HRP] Polyclonal Antibody (GenScript, A00160, 1:20,000) »

<
>

Target Background β-actin is one of the six different actin isoforms that have been identified.The actin molecules found in cells of various species and tissues tend to be very similar in their immunological and physical properties.As a consequence, it has been difficult to produce potent antisera against this protein.Therefore, the availability of monoclonal antibodies to ß-actin provides a useful and specific tool in the study of the intracellular distribution of β-actin and the static and dynamic aspects of the cytoskeleton.
Synonyms cytoplasmic 1 antibody;
返回

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


Qian Du, et al. Porcine circovirus type 2 infection promotes the SUMOylation of nucleophosmin-1 to facilitate the viral circular single-stranded DNA replication. PLoS Pathog. (2024-02)
Chao Wang , et al. Mechanosensitive accumulation of non-muscle myosin IIB during mitosis requires its translocation activity. iScience . (2023-08)
Sheikh Tahir Majeed, et al. MTORC2 is a physiological hydrophobic motif kinase of S6 Kinase 1. Biochim Biophys Acta Mol Cell Res. (2023-02)
Museer A Lone, et al. SPTLC1 variants associated with ALS produce distinct sphingolipid signatures through impaired interaction with ORMDL proteins. J Clin Invest. (2022-09)
Shashank Kumar Maurya, et al. Co-expression and Interaction of Pax6 with Genes and Proteins of Immunological Surveillance in the Brain of Mice. Neurotox Res. (2022-09)
Yuning Liu, et al. Hyperactivity of Purkinje cell and motor deficits in C9orf72 knockout mice. Mol Cell Neurosci. (2022-07)
Qian Zhou, et al. Phenylalanine impairs insulin signaling and inhibits glucose uptake through modification of IRβ. Nat Commun. (2022-07)
Evert J Loef, et al. Plasminogen and plasmin can bind to human T cells and generate truncated CCL21 that increases dendritic cell chemotactic responses. J Biol Chem. (2022-06)
Jinping Wang, et al. Osteopontin aggravates acute lung injury in influenza virus infection by promoting macrophages necroptosis. Cell Death Discov. (2022-03)
Zhenyu Wang, et al. Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR. Vet Microbiol. (2022-01)
Zhenyu Wang, et al. Porcine circovirus type 2 infection inhibits the activation of type I interferon signaling via capsid protein and host gC1qR. Vet Microbiol. (2022-01)
Mari J Aaltonen, et al. Serine palmitoyltransferase assembles at ER-mitochondria contact sites. Life Sci Alliance. (2021-11)
Jun Zheng, et al. Ubiquitin ligase MARCH5 localizes to peroxisomes to regulate pexophagy. J Cell Biol. (2021-11)
Pengjie Hu, et al. A unique cell wall synthetic response evoked by glucosamine determines pathogenicity-associated fungal cellular differentiation. PLoS Genet. (2021-10)
Weixiang Bian, et al. Low-density-lipoprotein-receptor-related protein 1 mediates Notch pathway activation. Dev Cell. (2021-10)
Tong Zhu, et al. Ethylene-induced NbMYB4L is involved in resistance against tobacco mosaic virus in Nicotiana benthamiana. Mol Plant Pathol. (2021-10)
Linda C Horianopoulos, et al. Dnj1 Promotes Virulence in by Maintaining Robust Endoplasmic Reticulum Homeostasis Under Temperature Stress. Front Microbiol. (2021-09)
Zhenyu Wang, et al. PCV2 targets cGAS to inhibit type I interferon induction to promote other DNA virus infection. PLoS Pathog. (2021-09)
Qiang Su, et al. Structural basis for Ca activation of the heteromeric PKD1L3/PKD2L1 channel. Nat Commun. (2021-08)
Jianxiong Guo, et al. Transmissible gastroenteritis virus ORF3b up-regulates miR-885-3p to counteract TNF-α production via inhibiting NF-κB pathway. Vet Microbiol. (2021-07)
Y B Liu, et al. Directed evolution of AAV accounting for long-term and enhanced transduction of cardiovascular endothelial cells. Mol Ther Methods Clin Dev. (2021-06)
Xingchen Wu, et al. Porcine circovirus type 2 infection attenuates the K63-linked ubiquitination of STING to inhibit IFN-β induction via p38-MAPK pathway. Vet Microbiol. (2021-05)
Yinsuo Zhao, et al. Metaxins are core components of mitochondrial transport adaptor complexes. Nat Commun. (2021-01)
Li Y, et al. Dual functions of Rack1 in regulating Hedgehog pathway. Cell death and differentiation. (2020-11)
Xin JW, et al. Signature of high altitude adaptation in the gluteus proteome of the yak. Journal of experimental zoology. Part B, Molecular and developmental evolution. (2020-09)
Cheng ZL, et al. The Zscan4-Tet2 Transcription Nexus Regulates Metabolic Rewiring and Enhances Proteostasis to Promote Reprogramming. Cell Reports. (2020-07)
Takushi SE, et al. Lentiviral Gene Therapy for Familial Hemophagocytic Lymphohistiocytosis Type 3, Caused by UNC13D Genetic Defects. Human gene therapy. (2020-06)
Xu N, et al. The Pyrimidine Analog FNC Potently Inhibits the Replication of Multiple Enteroviruses. Journal of Virology. (2020-04)
Wu J, et al. CDK9 inhibitors reactivate p53 by downregulating iASPP. Cell Signal. (2020-03)
Matthew Hackbart, et al. Coronavirus endoribonuclease targets viral polyuridine sequences to evade activating host sensors. PNAS. (2020-03)
He W, et al. Silver nanoparticles stimulate osteogenesis of human mesenchymal stem cells through activation of autophagy. Nanomedicine (Lond). (2020)
Oum KaltoumHassaniaVladimir V.RymaraKhanh Q.NguyenaLiaHuoaJean-FrançoisCloutieraFreda D.MillerbAbbas F.Sadikota, et al. The noradrenergic system is necessary for survival of vulnerable midbrain dopaminergic neurons: implications for development and Parkinson's disease. ScienceDirect. (2020)
Dimori M, et al. Respiratory defects in the CrtapKO mouse model of Osteogenesis Imperfecta. Am J Physiol Lung Cell Mol Physiol. (2020)
Loef EJ,, Brooks AES,, Lorenz N,, Birch NP,, Dunbar PR,., et al. Neuroserpin regulates human T cell-T cell interactions and proliferation through inhibition of tissue plasminogen activator. J Leukoc Biol. (2020)
Qu YY,,, Zhao R, Zhang HL,, Zhou Q, Xu FJ,, Zhang X, Xu WH,, Shao N,, Zhou SX,, Dai B,, Zhu Y,, Shi GH,, Shen YJ,, Zhu YP,, Han CT,, Chang K,, Lin Y,,, Zang WD, Xu W,,, Ye DW,, Zhao SM,,, Zhao JY,,., et al. Inactivation of the AMPK-GATA3-ECHS1 Pathway Induces Fatty Acid Synthesis That Promotes Clear Cell Renal Cell Carcinoma Growth. Cancer Res. (2020)
Mettelman RC, et al. Generating and evaluating type I interferon receptor-deficient and feline TMPRSS2-expressing cells for propagating serotype I feline infectious peritonitis virus. Virology. (2019-11)
RathCarolina Torturella, et al. Amazonian Phlebovirus (Bunyaviridae) potentiates the infection of Leishmania (Leishmania) amazonensis: Role of the PKR/IFN1/IL-10 axis. PLoS Negl Trop Dis. (2019-06)
JangHyo Sik, et al. Author Correction: Transposable elements drive widespread expression of oncogenes in human cancers. Nat. Genet. (2019-05)
AdamsJessie, et al. Autophagy-lysosome pathway alterations and alpha-synuclein up-regulation in the subtype of neuronal ceroid lipofuscinosis, CLN5 disease. Sci Rep. (2019-01)
WangHongjie, et al. RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain. PLoS ONE. (2019-01)
AdhikariBabita, et al. Neuronal ceroid lipofuscinosis related ER membrane protein CLN8 regulates PP2A activity and ceramide levels. Biochim Biophys Acta Mol Basis Dis. (2019)
CrowleyNicole A, et al. Ketamine normalizes binge drinking-induced defects in glutamatergic synaptic transmission and ethanol drinking behavior in female but not male mice. Neuropharmacology. (2019)
Jang HS,, Shah NM,, Du AY,, Dailey ZZ,, Pehrsson EC,, Godoy PM,, Zhang D,, Li D,, Xing X,, Kim S,, O'Donnell D,,, Gordon JI,,, Wang T,., et al. Transposable elements drive widespread expression of oncogenes in human cancers. Nat Genet. (2019)
Pandey S, et al. WT1 regulates cyclin A1 expression in K562 cells. Oncol Rep. (2019)
Wu X, et al. Porcine Circovirus Type 2 Rep Enhances IL-10 Production in Macrophages via Activation of p38-MAPK Pathway. Viruses. (2019)
WangHongjie, et al. Correction: RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain. PLoS ONE. (2019)
Zhao Z, et al. CAEV Vif Hijacks ElonginB/C, CYPA and Cullin5 to Assemble the E3 Ubiquitin Ligase Complex Stepwise to Degrade oaA3Z2-Z3. Front Microbiol. (2019)
Meyer KD1, et al. DART-seq: an antibody-free method for global m6A detection. Nat Methods. (2019)
WangZhen, et al. GPER stabilizes F-actin cytoskeleton and activates TAZ via PLCβ-PKC and Rho/ROCK-LIMK-Cofilin pathway. Biochem. Biophys. Res. Commun. (2019)
SunFenglin, et al. Acacetin-induced cell apoptosis in head and neck squamous cell carcinoma cells: Evidence for the role of muscarinic M3 receptor. Phytother Res. (2019)
Hsieh CH1, et al. Miro1 Marks Parkinson's Disease Subset and Miro1 Reducer Rescues Neuron Loss in Parkinson's Models. Cell Metab. (2019)
Zhang X, et al. Autophagy Promotes Porcine Parvovirus Replication and Induces Non-Apoptotic Cell Death in Porcine Placental Trophoblasts. Viruses. (2019)
YoshidaYukari, et al. X-ray irradiation induces disruption of the blood-brain barrier with localized changes in claudin-5 and activation of microglia in the mouse brain. Neurochem. Int. (2018-10)
BanerjeeSomanshu, et al. Specific neural phase relation of serotonin and dopamine modulate the testicular activity in Japanese quail. J. Cell. Physiol. (2018-08)
Balcik-ErcinPelin, et al. Genome-wide analysis of endogenously expressed ZEB2 binding sites reveals inverse correlations between ZEB2 and GalNAc-transferase GALNT3 in human tumors. Cell Oncol (Dordr). (2018-08)
MayorgaLía, et al. A frame-shift deletion in the PURA gene associates with a new clinical finding: Hypoglycorrhachia. Is GLUT1 a new PURA target?. Mol. Genet. Metab. (2018-03)
CohenJustin, et al. HIV antiretroviral therapy drugs induce premature senescence and altered physiology in HUVECs. Mech. Ageing Dev. (2018-01)
Banerjee S, et al. Simulated photoperiod influences testicular activity in quail via modulating local GnRHR-GnIHR, GH-R, Cnx-43 and 14-3-3. J Photochem Photobiol B. (2018-01)
Fan R, et al. SOX7 Suppresses Wnt Signaling by Disrupting β-Catenin/BCL9 Interaction. DNA Cell Biol. (2018)
Butler Kameryn M, et al. De novo variants in GABRA2 and GABRA5 alter receptor function and contribute to early-onset epilepsy. Brain. (2018)
Sen U, et al. Plasmodium falciparum RUVBL3 protein: a novel DNA modifying enzyme and an interacting partner of essential HAT protein MYST. Sci Rep. (2018)
Shu Q, et al. Protective effects of Progranulin against focal cerebral ischemia-reperfusion injury in rats by suppressing endoplasmic reticulum stress and NF-κB activation in reactive astrocytes. J Cell Biochem. (2018)
Wang D, et al. Colonic Lysine Homocysteinylation Induced by High-Fat Diet Suppresses DNA Damage Repair. Cell Rep. (2018)
Kang D, et al. The InR/Akt/TORC1 Growth-Promoting Signaling Negatively Regulates JAK/STAT Activity and Migratory Cell Fate during Morphogenesis. Dev Cell. (2018)
Shahin Saba, et al. From the Cover: 2.45-GHz Microwave Radiation Impairs Hippocampal Learning and Spatial Memory: Involvement of Local Stress Mechanism-Induced Suppression of iGluR/ERK/CREB Signaling. Toxicol. Sci. (2018)
AbrahamMabel N, et al. Cecal Ligation and Puncture Alters Glucocorticoid Receptor Expression. Crit. Care Med. (2018)
He Xia-Di, et al. Sensing and Transmitting Intracellular Amino Acid Signals through Reversible Lysine Aminoacylations. Cell Metab. (2018)
HeChun-Yan, et al. Inhibition of NF-κB by Pyrrolidine Dithiocarbamate Prevents the Inflammatory Response in a Ligature-Induced Peri-Implantitis Model: A Canine Study. Cell. Physiol. Biochem. (2018)
LiuChang-Yun, et al. DL-3-n-butylphthalide protects endothelial cells against advanced glycation end product-induced injury by attenuating oxidative stress and inflammation responses. Exp Ther Med. (2017-09)
Deng X, et al. Coronavirus nonstructural protein 15 mediates evasion of dsRNA sensors and limits apoptosis in macrophages. Proceedings of the National Academy of Sciences of the United States of America. (2017-05)
Duivenvoorden WCM, et al. Endoplasmic reticulum protein ERp46 in prostate adenocarcinoma. Oncol Lett. (2017-05)
Xufang Deng, et al. Coronavirus Nonstructural Protein 15 Mediates Evasion of dsRNA Sensors and Limits Apoptosis in Macrophages. Proc Natl Acad Sci U S A. (2017-04)
Lenis TL, et al. Complement modulation in the retinal pigment epithelium rescues photoreceptor degeneration in a mouse model of Stargardt disease. Proc Natl Acad Sci U S A. (2017-04)
Deb DK, et al. Critical role of the cAMP-PKA pathway in hyperglycemia-induced epigenetic activation of fibrogenic program in the kidney. FASEB J. (2017-02)
CohenJustin, et al. Astrocyte Senescence and Metabolic Changes in Response to HIV Antiretroviral Therapy Drugs. Front Aging Neurosci. (2017-01)
Lin Ran, et al. CLOCK Acetylates ASS1 to Drive Circadian Rhythm of Ureagenesis. Mol. Cell. (2017)
Melero-Fernandez de Mera Raquel M, et al. A simple optogenetic MAPK inhibitor design reveals resonance between transcription-regulating circuitry and temporally-encoded inputs. Nat Commun. (2017)
Bogenberger James, et al. Combined venetoclax and alvocidib in acute myeloid leukemia. Oncotarget. (2017)
Dilip K. Deb, et al. ATP-citrate lyase is essential for high glucose-induced histone hyperacetylation and fibrogenic gene up-regulation in mesangial cells. American Journal of Physiology-Renal Physiology. (2017)
Wang Z,, Liu P,, Zhou X,, Wang T, Feng X,, Sun YP, Xiong Y,, Yuan HX, Guan KL,., et al. Endothelin Promotes Colorectal Tumorigenesis by Activating YAP/TAZ. Cancer Res. (2017)
Hai-Tao Du, et al. Matrix metalloproteinase 14 participates in corneal lymphangiogenesis through the VEGF-C/VEGFR-3 signaling pathway. Exp Ther Med. (2016-08-01)
HeardMelissa E, et al. Sc65-Null Mice Provide Evidence for a Novel Endoplasmic Reticulum Complex Regulating Collagen Lysyl Hydroxylation. PLoS Genet. (2016)
ZhuSiying, et al. PERK Regulates Working Memory and Protein Synthesis-Dependent Memory Flexibility. PLoS ONE. (2016)
, et al. Phosphorylation of BMK1 induces prostatic carcinoma cell proliferation by promoting entry into the S phase of the cell cycle. ONCOLOGY LETTERS. (2016)
Zhang YK, et al. Enoyl-CoA hydratase-1 regulates mTOR signaling and apoptosis by sensing nutrients. Nat Commun. (2016)
CekanPavol, et al. RCC1-dependent activation of Ran accelerates cell cycle and DNA repair, inhibiting DNA damage-induced cell senescence. Mol. Biol. Cell. (2016)
Silva BD, et al. Proteolytic processing of the neuronal ceroid lipofuscinosis related lysosomal protein CLN5. Exp Cell Res. (2015-10)
Banerjee S, et al. Nesfatin-1: Localization and expression in avian gonads and its modulation by temporal phase relation of neural oscillations in female Japanese quail, Coturnix coturnix japonica. Gen Comp Endocrinol. (2015-08)
WangRuizhi, et al. COPS5 protein overexpression increases amyloid plaque burden, decreases spinophilin-immunoreactive puncta, and exacerbates learning and memory deficits in the mouse brain. J. Biol. Chem. (2015-04)
Deglincerti A, et al. Coupled local translation and degradation regulate growth cone collapse. Nat Commun. (2015)
Zhou J, et al. Ubiquitin E3 Ligase LNX2 is Critical for Osteoclastogenesis In Vitro by Regulating M-CSF/RANKL Signaling and Notch2. Calcif Tissue Int. (2015)
Zhou Z, et al. Stability of HIB-Cul3 E3 ligase adaptor HIB Is Regulated by Self-degradation and Availability of Its Substrates. Sci Rep. (2015)
Zhao D, et al. NOTCH-induced aldehyde dehydrogenase 1A1 deacetylation promotes breast cancer stem cells. J Clin Invest. (2014-12)
Y Liu, et al. Modulation of hepatitis B surface antigen secretion by annexin II expressed in hepatitis B virus-producing hepatoma cells. Mol Med Rep. (2014-12)
Chu YD, et al. RACK-1 regulates let-7 microRNA expression and terminal cell differentiation in Caenorhabditis elegans. Cell Cycle. (2014-12)
R Wang, et al. RanBP9 Overexpression Accelerates Loss of Dendritic Spines in a Mouse Model of Alzheimer's Disease. Neurobiol Dis. (2014-06)
H Wang, et al. RanBP9 Overexpression Accelerates Loss of Pre and Postsynaptic Proteins in the APΔE9 Transgenic Mouse Brain. PLoS One. (2014-01)
H Wang, et al. RanBP9 overexpression reduces dendritic arbor and spine density. Neuroscience. (2014-01)
Liu YM, et al. High expression of beta2-glycoprotein I is associated significantly with the earliest stages of hepatitis B virus infection. J Med Virol. (2014)
Murai Marcelo J, et al. The same site on the integrase-binding domain of lens epithelium-derived growth factor is a therapeutic target for MLL leukemia and HIV. Blood. (2014)
Hongjie Wang, et al. COPS5 (Jab1) increases β-site processing of amyloid precursor protein and Aβ generation by stabilizing RanBP9 protein levels. J Biol Chem. (2013-8)
Palavicini JP, et al. RanBP9 Overexpression Down-Regulates Phospho-Cofilin, Causes Early Synaptic Deficits and Impaired Learning, and Accelerates Accumulation of Amyloid Plaques in the Mouse Brain. J Alzheimers Dis. (2013-11)
Gruenwald K, et al. Sc65 is a novel endoplasmic reticulum protein that regulates bone mass homeostasis. J Bone Miner Res. (2013-08)
Palavicini JP, et al. RanBP9 aggravates synaptic damage in the mouse brain and is inversely correlated to spinophilin levels in Alzheimer’s brain synaptosomes. Cell Death Dis. (2013-06)
Hayes CD, et al. Striking reduction of amyloid plaque burden in an Alzheimer's mouse model after chronic administration of carmustine. BMC Med. (2013-03)
Hayes CD, et al. Chronic Cladribine Administration Increases Amyloid Beta Peptide Generation and Plaque Burden in Mice. PLoS One. (2012-10)
Cai Y, et al. BACE1 elevation is involved in amyloid plaque development in the triple transgenic model of Alzheimer's disease: differential Aβ antibody labeling of early-onset axon terminal pathology. Neurotox Res. (2012-02)
查看更多
返回