| ￥3800 | |
| Z03533-100 | |
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| Species | SARS-CoV-2 | ||||||
| Protein Construction |
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| Purity | > 90% as analyzed by SDS-PAGE | ||||||
| Endotoxin Level | < 0.2 EU/μg of protein by gel clotting method | ||||||
| Biological Activity | SARS-CoV-2 Spike protein (RBD, N501Y, Avi & His Tag) can bind with human ACE2 (Cat. No.: Z03484) in functional ELISA assay. | ||||||
| Expression System | 293 Cells | ||||||
| Theoretical Molecular Weight | 30 kDa | ||||||
| Formulation | Supplied as a solution in PBS, pH 7.4. | ||||||
| Concentration | Please refer to the COA for the specific lot. | ||||||
| Storage & Stability | Upon receiving, this product remains stable for 6 months at -20°C or below. Avoid repeated freeze-thaw cycles. |
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Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.2 μg/mL).
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Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag) (Cat. No. Z03533) with an affinity constant of 2.11 nM as determined in BLI assay. »
Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, N501Y, His & Avi Tag), reducing(R)
> 95% as analyzed by SDS-PAGE
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| Target Background | SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. The spike protein mutation N501Y is one of six key contact residues within the receptor-binding domain (RBD) and has been identified as increasing binding affinity to human and murine ACE2. |
| Synonyms | SARS-CoV-2 SP RBD, N501Y; 2019-nCoV SP RBD, N501Y |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
