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SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

Western blot analysis of SARS-CoV-2 Spike protein (RBD, His & Avi tag) (GenScript, Z03483) using S1 Antibody(GenScript, A02053).
Lane 1: 10 ng of Z03483
Lane 2: 5 ng of Z03483

SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

Western blot analysis of SARS-CoV-2 Spike protein (RBD, His & Avi tag) (GenScript, Z03483) using His Antibody(GenScript, A00186).
Lane 1: 10 ng of Z03483
Lane 2: 5 ng of Z03483

SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag) (Cat. No. Z03483) with a serial dilution.
THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.1 μg/mL).

SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag) (Cat. No. Z03483) with an affinity constant of 12.5 nM as determined in BLI assay.

SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
Lane 2: 3μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
Lane 3: 5μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
Lane 4: 5μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), non-reducing(NR)
> 95% as analyzed by SDS-PAGE

SARS-CoV-2 Spike protein (RBD, His & Avi tag)

SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus.
Z03483
￥3800

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产品简介
Species SARS-CoV-2
Protein Construction
Spike RBD (Arg319-Ser591)
Accession # P0DTC2		 Avi Poly-His
N-term C-term
Purity > 95% as analyzed by SDS-PAGE
Endotoxin Level < 0.2 EU/μg of protein by gel clotting method
Biological Activity SARS-CoV-2 Spike protein (RBD, Avi & His Tag) can bind with Human ACE2 in functional ELISA assay.
Expression System 293 Cells
Theoretical Molecular Weight 30 kDa
Formulation Supplied as a solution in PBS pH 7.2.
Concentration Please refer to the COA for the specific lot.
Storage & Stability Upon receiving, this product remains stable for up to 6 months at -20°C or below. Avoid repeated freeze-thaw cycles.

图例
  • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)
    • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

    Western blot analysis of SARS-CoV-2 Spike protein (RBD, His & Avi tag) (GenScript, Z03483) using S1 Antibody(GenScript, A02053).
    Lane 1: 10 ng of Z03483
    Lane 2: 5 ng of Z03483

  • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)
    • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

    Western blot analysis of SARS-CoV-2 Spike protein (RBD, His & Avi tag) (GenScript, Z03483) using His Antibody(GenScript, A00186).
    Lane 1: 10 ng of Z03483
    Lane 2: 5 ng of Z03483

  • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)
    • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

    Immobilized ACE-2 Fc Chimera, Human (Cat. No. Z03484) at 2 μg/mL can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag) (Cat. No. Z03483) with a serial dilution.
    THETM His Tag Antibody [HRP], mAb, Mouse (Cat.No.A00612) is used as a secondary antibody (0.1 μg/mL).

  • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)
    • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

    Loaded ACE-2 Fc Chimera, Human (CHO-expressed) Protein (Cat. No. Z03516) on Protein A Biosensor, can bind SARS-CoV-2 Spike protein (RBD, His & Avi Tag) (Cat. No. Z03483) with an affinity constant of 12.5 nM as determined in BLI assay.

  • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)
    • SARS-CoV-2 Spike Protein (RBD, His & Avi Tag)

    Lane 1: 1μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
    Lane 2: 3μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
    Lane 3: 5μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), reducing(R)
    Lane 4: 5μg of SARS-CoV-2 Spike protein (RBD, His & Avi tag), non-reducing(NR)
    > 95% as analyzed by SDS-PAGE


技术背景
Target Background SARS-CoV-2 (Severe Acute Respiratory Syndrome Coronavirus 2) also known as 2019-nCoV (2019 Novel Coronavirus) is a virus that causes illnesses ranging from the common cold to severe diseases. SARS-CoV-2 Spike Protein is composed of S1 domain and S2 domain. S1 contains a receptor-binding domain (RBD) that can specifically bind to angiotensin-converting enzyme 2 (ACE2), the receptor on target cells. It is believed that SARS-CoV-2 Spike Protein (RBD) has potential value for the diagnosis of the virus.
Synonyms SARS-CoV-2 SP RBD; 2019-nCoV SP RBD

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.


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