|Synonyms||Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.|
|Description||Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.
Recombinant Porcine IL-1β produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
|Biological Activity||ED50 < 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 106 IU/mg.|
Ala115-Pro267 (Accession #:P26889), expressed with an N-terminal Met
|Measured Molecular Weight||17.7 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.|