• 中文
  • 登录
  • 联系我们
×
目录产品 » 重组蛋白 » 细胞因子 » Interleukin-1 beta (IL-1β),  Porcine

IL-1β, Porcine

Synonyms Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.
Description Interleukin-1 beta (rpIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. IL-1α and IL-1β are structurally related polypeptides that share approximately 27% amino acid (aa) identity in porcine. While IL-1α and IL-1β are regulated independently, they bind to the same receptor and exert identical biological effects. IL-1β stimulates thymocyte proliferation by inducing il-2 release, b-cell maturation and proliferation, and fibroblast growth factor activity.
Recombinant Porcine IL-1β produced in E. coli is a single non-glycosylated polypeptide chain containing 154 amino acids. A fully biologically active molecule, rpIL-1β has a molecular mass of 17.7 kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No P26889
Source E. coli
Biological Activity ED50 < 1ng/ml, measured by the dose-dependent stimulation of mouse D10S cells, corresponding to a specific activity of 1.0× 106 IU/mg.
Sequence
Ala115-Pro267 (Accession #:P26889), expressed with an N-terminal Met

Measured Molecular Weight 17.7 kDa, observed by reducing SDS-PAGE.
Purity > 95% as analyzed by SDS-PAGE.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O or PBS at 100 μg/ml.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Porcine IL-1β, remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, Porcine IL-1β should be stable up to 1 week at 4°C or up to 3 months at -20°C.




请点击此处查找更多相关文献







更多产品文件类型 >>

产品编号: Z03278
规格
价格
Z03278-10 10 ug ¥700.00
Z03278-50 50 ug ¥1500.00
Z03278-1 1 mg ¥14000.00