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目录产品 » 重组蛋白 » 细胞因子 » Hedgehog homolog (DHH), Human

DHH, Human

Synonyms DHH, Human
Description Desert hedgehog protein (DHH) is a member of the Hedgehog family which encodes signaling molecules that play an important role in regulating morphogenesis. It is predicted to be made as a precursor that is auto-catalytically cleaved; the N-terminal portion is soluble and contains the signaling activity while the C-terminal portion is involved in precursor processing. More importantly, the C-terminal product covalently attaches a cholesterol moiety to the N-terminal product, restricting the N-terminal product to the cell surface and preventing it from freely diffusing throughout the organism. Defects in this protein have been associated with partial gonadal dysgenesis (PGD) accompanied by minifascicular polyneuropathy. DHH may be involved in both male gonadal differentiation and perineurial development. DHH binds both Patched and Patched 2 as well as Hedgehog interacting protein (Hip). It induces steroidogenic factor 1(SF1), which is instrumental in promoting Leydig cell differentiation. It also promotes the deposition of basal lamina surrounding seminiferous tubules.
Recombinant human Desert hedgehog (DHH) produced in Escherichia Coli is a single non-glycosylated polypeptide chain containing 177 amino acids. A fully biologically active molecule, rhDHH has a molecular mass of around 20kDa analyzed by reducing SDS-PAGE and is obtained by chromatographic techniques at GenScript.
Accession No O43323
Species Human
Source E. coli
Biological Activity ED50 < 10μg/, measured by its ability to induce alkaline phosphatase production by CCL-226 cells, corresponding to a specific activity of > 100 units/mg.
Sequence
IIGPGRGPVG RRRYARKQLV PLLYKQFVPG VPERTLGASG
PAEGRVARGS ERFRDLVPNY NPDIIFKDEE NSGADRLMTE
RCKERVNALA IAVMNMWPGV RLRVTEGWDE DGHHAQDSLH
YEGRALDITT SDRDRNKYGL LARLAVEAGF DWVYYESRNH
VHVSVKADNS LAVRAGG

Measured Molecular Weight ~20 kDa, observed by reducing SDS-PAGE.
Purity > 95% by SDS-PAGE and HPLC analyses.
Formulation Lyophilized after extensive dialysis against PBS.
Reconstitution Reconstituted in ddH2O at 100 μg/mL.
Endotoxin Level < 0.2 EU/μg, determined by LAL method.
Storage Lyophilized recombinant Human Hedgehog homolog(rhDHH) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhDHH remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.
Note For research use only




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产品编号: Z03234
规格
价格
1137107 Z03234-10 10 ug ¥600.00
1137108 Z03234-50 50 ug ¥1200.00
1137041 Z03234-1 1 mg ¥8800.00