|Synonyms||NKSF, CTL maturation factor (TCMF), Cytotoxic lymphocyte maturation factor (CLMF), TSF, Interferon-gamma-inducing factor, IGIF, IL-1g, IL1F4, IL-1 gamma|
|Description||Interleukin-18 (IL18, also known as interferon-gamma inducing factor or IGIF) is a cytokine that belongs to the IL-1 superfamily and is produced by macrophages and other cells. Its biological activity is pleiotropic and it has been shown to induce interferon-gamma production in KG-1 cells. The combination of this cytokine and IL12 has been shown to inhibit IL4 dependent IgE and IgG1 production, and enhance IgG2a production in B cells.
Recombinant rat Interleukin-18 (rrIL-18) produced in E. coli is a single non-glycosylated polypeptide chain containing 159 amino acids. A fully biologically active molecule, rrIL-18 has a molecular mass of 18.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 <2μg/ml resulted in the secretion of 0.15 ng/ml h-IFN-gamma when tested using KG-1 cell line, corresponding to a specific activity of > 500 units/mg.|
MHFGRLHCTT AVIRSINDQV LFVDKRNPPV FEDMPDIDRT
ANESQTRLII YMYKDSEVRG LAVTLSVKDG RMSTLSCKNK
IISFEEMNPP ENIDDIKSDL IFFQKRVPGH NKMEFESSLY
EGHFLACQKE DDAFKLVLKR KDENGDKSVM FTLTNLHQS
|Measured Molecular Weight||18.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analyses.|
|Formulation||Lyophilized after extensive dialysis against 50mM Tris, 50mM NaCl, pH8.0.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant ratInterleukin-18 (rrIL-18) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rrIL-18 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|