MonoRab™ Anti-VHH Affinity Magnetic Beads

This Microbead is for purifying camelid and humanized VHH antibodies.

This product is specifically designed for purifying camelid and humanized VHH from commonly used protein expression systems such as bacteria, yeast, and mammalian cells.

价格	￥3800 在线订购包邮(港澳台除外)
产品编号	L01034-1
规格	2 ml (1 ml Settled Beads) 10 ml (5 ml Settled Beads) 20 ml (10 ml Settled Beads) 50 ml (25 ml Settled Beads) 大单询价
数量
参考货期


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产品概述产品属性图例

产品概述

Product Description

VHH, also known as nanobodies, are the smallest functional fragments of naturally occurring heavy-chain antibodies found in camelids. Their compact size, high stability, and solubility make them valuable tools for various applications in biotechnology, diagnostics, and therapeutics. Besides, when VHH are intended for use in humans, it is often necessary to humanize them to reduce their immunogenicity and enhance their compatibility with the human immune system. Therefore, the purification of both humanized and camelid VHH are crucial. GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034) is specifically designed for purifying camelid and humanized VHH from commonly used protein expression systems such as bacteria, yeast, and mammalian cells. The VHH in cell lysate can specifically bind to the anti-VHH antibody coupled to the beads. Non-specific binding reactions are eliminated through rigorous washing steps, and the purified target protein is eluted with a high recovery rate.

产品属性

Ligand	MonoRab™ Anti-VHH Antibody
Binding capacity	≥2 mg VHH per mL settled beads (depending on amino acid sequence of different VHHs)
Matrix	Magnetic agarose beads
Average particle size	10-37 μm
Storage & Stability	Store at 2-8 °C for 12 months. Do not freeze the beads

图例

Regeneration performance of MonoRab™ Anti-VHH Affinity Magnetic Beads

The target VHH was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The 100 μL settled beads were washed twice with 1 mL TBS and then eluted with 1 mL acidic eluent (0.1 M glycine HCl, pH 2.5). Subsequently, the beads were regenerated by 1 mL regeneration buffer for reuse. The coefficient of variation (CV) of the amount of eluted target protein was less than 20% for a total of 10 replicate purifications indicating that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads is capable of multiple repeated use with relatively high consistency when eluted by acidic eluent. »

Purification of humanized VHH from E.coli whole cell lysate

The humanized VHH (M.W.=15 kDa) from E.coli whole cell lysate was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The captured humanized VHH was subsequently eluted using an acidic eluent (0.1 M glycine HCl, pH 2.5) and subjected to SDS-PAGE analysis. The results showed that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads are suitable for high-specificity purification of humanized VHH from E.coli whole cell lysate
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
Lane 2: E.coli whole cell lysate containing humanized VHH
Lane 3: Flow-through of E.coli whole cell lysate containing humanized VHH
Lane 4: Eluate of target humanized VHH from E.coli whole cell lysate with L01034
»

Purification of camelid VHH from various lysates

The camelid VHH (M.W.=15 kDa ) from E.coli whole cell lysate, yeast whole cell lysate or mammalian cell culture supernatant was captured by GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads (Cat. No. L01034). The captured camelid VHH was subsequently eluted using an acidic eluent (0.1 M glycine HCl, pH 2.5) and subjected to SDS-PAGE analysis. The results showed that GenScript’s MonoRab™ Anti-VHH Affinity Magnetic Beads are suitable for high-specificity purification of camelid VHH from widely used protein expression systems.
Lane 1: Broad Multi Color Pre-Stained Protein Standard (GenScript, M00624)
E.coli：
Lane 2: E.coli whole cell lysate containing camelid VHH
Lane 3: Flow-through of E.coli whole cell lysate containing camelid VHH
Lane 4: Eluate of target camelid VHH from E.coli whole cell lysate with L01034
Yeast：
Lane 5: Yeast whole cell lysate containing camelid VHH
Lane 6: Flow-through of yeast whole cell lysate containing camelid VHH
Lane 7: Eluate of target camelid VHH from yeast whole cell lysate with L01034
Mammalian：
Lane 8: Mammalian cell culture supernatant containing camelid VHH
Lane 9: Flow-through of mammalian cell culture supernatant containing camelid VHH
Lane 10: Eluate of target camelid VHH from mammalian cell culture supernatant with L01034 »