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6X Loading Buffer with Agarose Gel Loading Dye

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This dye is used as a loading dye for DNA/RNA samples and DNA markers in agarose gels. It contains Bromophenol Blue and Xylene cyanol as tracking dye during electrophoresis. The EDTA is included in the solution to protect sample from nuclease degradation.Glycerol60% Tris-HCl (pH 7.6)10 mMEDTA 60 mMBromophenol Blue 0.03%Xylene Cyanol FF 0.03%
D0109
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Description

This dye is used as a loading dye for DNA/RNA samples and DNA markers in agarose gels. It contains Bromophenol Blue and Xylene cyanol as tracking dye during electrophoresis. The EDTA is included in the solution to protect sample from nuclease degradation.

Glycerol60%
Tris-HCl (pH 7.6)10 mM
EDTA 60 mM
Bromophenol Blue 0.03%
Xylene Cyanol FF 0.03%

QC
DNase RNaseNot detected
Stability 2-8 °C 12 months
Absorbance of Solution
(dilution 1:200 with deionized water)
at 422 nm ~ 0.03
at 615 nm ~ 0.35

Storage The product can be stored for 12 months at room temperature or 2-8°C and stored at -20°C for longer periods.

DNA sample preparation for agarose or polyacrylamide gel analysis.

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