Pembrolizumab is a humanized antibody used in cancer immunotherapy that targets the programmed cell death 1 (PD-1) receptor. It blocks a protective mechanism on cancer cells, and allows the immune system to destroy those cancer cells. The drug was initially used to treat metastatic melanoma.
Pembrolizumab ELISA Detection Kit is a sandwich enzyme-linked immunoassay which can be used for quantitative detection of Pembrolizumab drug in samples. The Capture Plate is pre-coated with an Anti- Pembrolizumab Monoclonal Antibody. When free Pembrolizumab or specimen is added to the well, the Pembrolizumab will be captured by the plate. Other unbound molecules are removed by the wash step. Then the Biotin-labeled detection antibody and Horseradish peroxidase conjugated streptavidin are added to react with the TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. The color developed is proportional to the amount of Pembrolizumab present in the testing samples. In optimized test conditions, each absorbance value is indicated to the individual Pembrolizumab amount in samples. The Pembrolizumab standards of known concentration and corresponding absorbance values are used to form a standard curve. With the standard curve, Pembrolizumab amount present in the unknown sample is calculated by transforming its absorbance value. |
