GenScript cGMP ELISA Detection Kit is a competition enzyme-linked immunoassay which can be used for quantitative detection of cGMP (Guanosine 3',5'-cyclic monophosphate) in samples such as serum, plasma, saliva, cell culture supernatant, and urine. cGMP is an important secondary messenger in signal transduction pathways. It is a common regulator of ion channel conductance, glycogenolysis, and cellular apoptosis. cGMP is a cyclic nucleotide derived from guanosine triphosphate (GTP). The cellular production of cGMP is mediated by Guanylate cyclase (GC). It activates the cGMP-dependent protein kinases which in turn phosphorylate several downstream protein targets. cGMP has an effect on the regulation of cAMP levels by activating or inhibiting specific phosphodiesterases (PDEs).
The anti-IgG Capture Plate is pre-coated with fixed amount of Goat anti-rabbit IgG to capture Rabbit Anti-cGMP Polyclonal Antibody. When free cGMP or specimen and HRP-cGMP conjugate are added to the well, they compete in the solution to interact with the cGMP antibody captured on the plate. Other unbound molecules are removed by a wash step. The cGMP-HRP reacts with TMB substrate to develop a blue product in the solution. The reaction is stopped by adding stop solution and the color turns yellow which can be read at 450 nm by a Microtiter plate reader. Using the standard curve, the amount of cGMP present in the unknown samples can be calculated by transforming its absorbance value.
Quantitative detection of cGMP in samples such as serum, plasma, saliva, cell culture supernatant, and urine.
The unopened kit is stable for at least 12 months if stored at 2-8 °C, and the opened kit is stable for up to 2 weeksat 2-8 °C. Do not freeze the kit.
|Storage||Store at 4°C|