Interleukin-8 (IL-8), a member of a family of structurally related cytokines, is a neutrophil chemotactic cytokine that can signal
through the CXCR1 and CXCR2 receptor. It is proinflammatory and primarily mediates the activation and migration of neutrophils from
peripheral blood into the sites of inflammation, injury, or infection in the tissue. IL-8 is involved in a wide variety of
physiological and pathological processes, including host defense against bacterial infection, bronchiolitis, arteriosclerosis,
autoimmune disorders of skin, and angiogenesis-dependent disorders such as tumor growth, and wound repair. IL-8 is produced by
stimulated monocytes, leukocytic cells, non-leukocytic somatic cells and tumor cells.
GenScript Human IL-8 ELISA Detection
Kit is a solid phase sandwich ELISA for quantitative measurement of human IL-8 in serum, plasma and cell culture supernates. It
utilizes a mouse anti-human IL-8 monoclonal antibody coated on 96-well plate as capture antibody. Standards and samples are
pipetted into the wells and the IL-8 present is bound by the immobilized antibody. After washing away any unbound substances,
another mouse anti-Human IL-8 monoclonal Antibody with HRP conjugation as detection antibody to specific bind human IL-8. After
washing again, TMB substrate solution is added and the color developed is proportional to the amount of IL-8 present in the testing
- Sensitivity: 2.8 pg/ml
- Large assay range: from 25 pg/ml to 1600 pg/ml
- Highly reproducible results
- Cost saving
- Time Saving Protocol: complete test in 2.5 hours:
- Quantitate human IL-8 in cell culture supernates, plasma and serum
- The unopened kit is stable for 12 months stored at 2-8°C.
- The opened kit may be stored for up to 1 month at 2-8°C.
- Use only before expiration date.
- Do not freeze the kit.