Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb

GenScript Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb reacts with the Fab portion of human IgG but not with the Fc portion of human IgG. The monoclonal antibody has no cross-reactivity with mouse, rat, rabbit, chicken or goat immunoglobulins.

价格	￥990 在线订购包邮(港澳台除外)
产品编号	A01855-200
规格	200 μl 大单询价
数量
参考货期


咨询产品问题

产品概述应用指导产品属性图例技术背景

产品概述

Specificity	GenScript Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb reacts with the Fab portion of human IgG but not with the Fc portion of human IgG. The monoclonal antibody has no cross-reactivity with mouse, rat, rabbit, chicken or goat immunoglobulins.
Host Species	Mouse
Immunogen	Human IgG(H&L)
Conjugate	Horseradish peroxidase (HRP)

应用指导

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by primary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.Optimal working dilutions must be determined by the end user.

Application	Recommended Usage
Western Blot	1:5,000-1:20,000
ELISA	1:5,000-1:20,000
Immunohistochemistry (IHC)	User-optimized

产品属性

Form	Liquid
Storage Buffer	Supplied in PBS (pH 7.4), 1% BSA, 0.01% thimerosal and 50% glycerol.
Concentration	Not applicable
Storage Instructions	Store at -20°C. This product is stable for 1 year upon receipt, when handled and stored as instructed.
Purification	Protein A affinity column
Isotype	Mouse IgG1,κ
Clonality	Monoclonal
Clone ID	12H3C4A6
Note	GenScript can customize this product per customer's request including product size, buffer components, etc.

图例

Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], MAb

Figure 2. Comparison of Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854; Green line), and Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb (GenScript, A01855; Blue line) binding to the Fc region of human IgG using ELISA.
Coating antigen: ACE-2 Fc Chimera, Human (GenScript, Z03484), 2 µg/ml;
Mouse Anti-Human IgG Fc Antibody (50B4A9)[HRP], mAb (GenScript, A01854) and Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb (GenScript, A01855) dilution start from 2 µg/ml. »

Figure 1. ELISA analysis of Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb (GenScript, A01855) with various subclasses of human IgG.
The coating antigens are recombinant human IgG1, recombinant human IgG2, recombinant human IgG3, recombinant human IgG4, recombinant human IgG fab fragment and total human IgG. The coating concentration is 1 µg/ml.
Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb (GenScript, A01855) dilution start from 1 µg/ml. »

技术背景

Target Background

Generally, goat anti-human or donkey anti-human IgG polyclonal antibodies are used as the secondary antibodies to detect human IgG (H+L). However, since polyclonal antibodies can recognize many epitopes, the background and the cross reactivity in assays is usually high, making results hard to interpret. As a mouse monoclonal antibody, the unique GenScript Mouse Anti-Human IgG Fab Antibody (12H3C4A6)[HRP], mAb has a high specificity to the human IgG Fab portion and can be used to detect human antibodies which include the human IgG Fab portion.

Synonyms

Anti-Human IgG Fab Monoclonal Secondary Antibody (Min X Ms, Rt, Rb, Ch, Gt)

For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.