• 中文
  • 登录
  • 联系我们
×
目录产品 » 抗体产品 » 标签抗体 » THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse

THE™ DYKDDDDK Tag Antibody [FITC], mAb, Mouse

Synonyms THETM Anti-DYKDDDDK [FITC] Monoclonal Antibody (Mouse); THETM Anti-Flag [FITC] Monoclonal Antibody (Mouse);
Description GenScript THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse is FITC conjugated THETM DYKDDDDK Tag Antibody, mAb, Mouse (A000187) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of DYKDDDDK fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
Host Species Mouse
Conjugation Fluorescein (FITC)
Immunogen A synthetic peptide DYKDDDDK coupled to KLH
Purification Protein A affinity column

Subclass IgG2b, k
Clone ID 5A8E5
Concentration 0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide
Reconstitution Reconstitute the lyophilized product with deionized water (or equivalent) to make antibody concentration of 0.5 mg/ml.
Specificity GenScript THETM DYKDDDDK Tag Antibody [FITC], mAb, Mouse recognizes C-terminal, N-terminal, and internal DYKDDDDK tagged fusion proteins.
Storage The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.
Note Briefly centrifuge the vial on a tabletop centrifuge to dislodge any liquid in the container's cap because small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage.

Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.5-2 µg/ml
Flow cytometry: 0.5-2 µg for 1 x 106 cells
Other applications: User-optimized


Pumroy RA., et al. Nucleoporin Nup50 Stabilizes Closed Conformation Of Armadillo Repeat 10 In Importin 5. J Biol Chem. 2012 Jan;287(3):2022-31.

请点击此处查找更多相关文献




更多产品文件类型 >>

产品编号: A01632
规格
价格
3148 A01632 100 μg ¥1200