|Synonyms||THETM His Tag Antibody [FITC], mAb, Mouse;|
|Description||GenScript THETM His Tag Antibody [FITC], mAb, Mouse is an FITC conjugated THETM His Tag Antibody, mAb, Mouse (A00186) purified from mice ascites by Protein A affinity column. This product is suitable for detecting the expression level of His fusion proteins by various immunoassays.
Absorption Wavelength: 492 nm
Emission Wavelength: 518 nm
Fluorochrome/Protein Ratio: >3 moles FITC per mole antibody
|Immunogen||A synthetic peptide HHHHHH coupled to KLH|
|Purification||Protein A affinity column|
|Concentration||1 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.02% sodium azide|
|Reconstitution||Reconstitute the lyophilized antibody with 100 μl of deionized water to the final concentration of 1 mg/ml.|
|Specificity||GenScript THETM His Tag Antibody [FITC], mAb, Mouserecognizes C-terminal, N-terminal, and internal His tagged fusion proteins.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
Immunofluorescence (IF): 0.1-2 µg/ml
Flow cytometry: 0.01-0.1 µg/ml
Other applications: User-optimized