| Specificity |
GenScript Rabbit Anti-Histone H3 Polyclonal Antibody detects endogenous levels of human, mouse and rat Histone H3. |
| Host Species |
Rabbit |
| Immunogen |
KLH-coupled synthetic peptide from C-terminal of human Histone H3 (Swiss Prot: P68431). |
| Species Reactivity |
Human, mouse and rat |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Immunocytochemistry/Immunofluorescence (ICC/IF) |
2-5 µg/ml |
| Western Blot |
0.5-1 µg/ml |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide. |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Target Background |
Histone H3 is the core component of nucleosome. It has been implicated in nucleosomes wrap and compacts DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.GenScript Rabbit Anti-Histone H3 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human Histone H3 (Swiss Prot: P68431). |
| Synonyms |
Rabbit Anti-Histone H3 polyclonal antibody; Histone H3 antibody |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
