| Specificity |
GenScript Rabbit Anti-mGluR5 Polyclonal Antibody detects endogenous levels of human mGluR5. It is predicted to react with mouse and rat mGluR5 protein according to sequence homology. Positive Control: Ramos |
| Host Species |
Rabbit |
| Immunogen |
KLH-coupled synthetic peptide from C-terminal of human mGluR5 (Swiss Prot: P41594) |
| Species Reactivity |
Human |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| Immunohistochemistry (IHC) |
5-10 µg/ml |
| Western Blot |
1-2 µg/ml |
| Flow Cytometry |
1-3 µg for 1 x 106 cells |
|
| Form |
Lyophilized |
| Storage Buffer |
lyophilized with PBS, pH 7.4, containing 0.02% sodium azide |
| Reconstitution |
Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/mL. |
| Storage Instructions |
The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Purification |
Affinity chromatography |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volumes of antibody will occasionally become entrapped in the seal of the product vial during shipment and storage. Before use, briefly centrifuge the vial to dislodge any liquid in the container's cap. |
| Target Background |
Metabotropic glutamate receptor 5 (mGluR5) is one member of G-protein coupled receptor 3 family. The activity of mGluR5 receptor is mediated by a G-protein that activates phosphatidylinositol-calcium second messenger system and generates calcium-activated chloride current. mGluR5 plays a critical role in the modulation of synaptic plasticity and behavioral responses to multiple substances of abuse. The expression of mGluR5 is widely distributed in brain regions relating to memory, such as the hippocampus. mGluR5 regulates phospholipid hydrolysis in developing cortical neurons through the activation of PLC-β1. The receptor regulates glutamatergic transmission in a variety of brain regions, particularly those thought to participate in reward-related behaviors. Its activation by the group I mGluR agonist (RS)-3,5-dihydroxyphenylglycine (DHPG) can induce long term depression (DHPG-LTD) of glutamatergic synaptic transmission.GenScript Rabbit Anti-mGluR5 Polyclonal Antibody is developed in rabbit using a KLH-coupled synthetic peptide from C-terminal of human mGluR5 (Swiss Prot: P41594). |
| Synonyms |
Rabbit Anti-mGluR5 Polyclonal Antibody; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
