THE™ DYKDDDDK Tag Antibody [HRP], mAb, Mouse
| Synonyms | Anti Flag-tag mAb; Anti DYKDDDDK-tag mAb |
| Description | GenScript THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse is HRP conjugated THETM Anti-DYKDDDDK Monoclonal Antibody (Mouse) (A00187) purified from mice ascites by protein A affinity column. The antibody is suitable for detecting the expression level of DYKDDDDK in fusion proteins. |
| Host Species | Mouse |
| Conjugation | Peroxidase (Horseradish) |
| Immunogen | A synthetic peptide (DYKDDDDK) coupled to KLH |
| Fusion Partner | Spleen cells were fused with SP2/0-Ag14 mouse myeloma cells. |
| Purification | Protein A affinity column |
| Subclass | IgG2b, k |
| Clone ID | 5A8E5 |
| Concentration | 0.5 mg/ml,
lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal. |
| Reconstitution | Reconstitute the lyophilized powder with deionized water (or equivalent) to an final concentration of 0.5 mg/ml. |
| Specificity | THETM DYKDDDDK Tag Antibody [HRP], mAb, Mouse recognizes C-terminal, N-terminal, and internal tagged fusion proteins. |
| Storage | The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles. |
| Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. ELISA: 0.05-0.2 µg/ml Western blot: 0.1-1.0 µg/ml Other applications: user-optimized |
Fan Y, et al. Profilin-1 phosphorylation directs angiocrine expression and glioblastoma progression through HIF-1α accumulation. Nat Cell Biol. 2014May;16(5):445-56
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| 产品编号: A01428 | ||
|---|---|---|
规格 |
价格 |
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| 100 μg | ¥1200 | |
