|Synonyms||Rabbit anti NWSHPQFEK [HRP] pAb;|
|Description||Well-characterized antibodies for epitope tags consisting of short sequences have been widely used in the study of protein expression in various systems.
The NWSHPQFEK, a nine-amino acid peptide that is capable of binding to the biotin pocket of streptavidin was engineered. The Rabbit anti NWSHPQFEK polyclonal antibody is developed in rabbit using a synthetic NWSHPQFEK peptide conjugated to KLH.
This polyclonal antibody is highly purified from rabbit antiserum by immunoaffinity chromatography and then conjugated to horseradish peroxidase. The antibody is suitable for detecting fusion proteins that contain NWSHPQFEK.
|Immunogen||Strep-tag II epitope peptide NWSHPQFEK conjugated to KLH.|
|Concentration||0.5 mg/ml, lyophilized with PBS, pH 7.4, containing 1% BSA and 0.01% thimerosal|
|Reconstitution||Reconstitute the lyophilized powder with deionized water (or equivalent) to an antibody concentration of 0.5 mg/ml.|
|Specificity||This Antibody recognizes Strep-tag II tagged fusion proteins.|
|Storage||The antibody is stable in lyophilized form if stored at -20°C or below. The reconstituted antibody can be stored for 2-3 weeks at 2-8°C. For long term storage, aliquot and store at -20°C or below. Avoid repeated freezing and thawing cycles.|
|Working concentrations for specific applications should be determined by the investigator. The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product.
ELISA: 0.05-0.2 µg/ml
Western blot: 0.5-1.0 µg/ml
Other Applications: user-optimized