| Specificity |
The specificity of antiserum is defined as the ratio of antigen concentration to cross-reactant concentration at 50% inhibition of maximum binding.The cross-reactivity data obtained in competitive ELISA system is as follows:table border Compound % Cross-reactivitycAMP 100AMP 0.0140cGMP 0.0290GMP 0.0223ADP 0.0069GDP 0.0011ATP <0.0001GTP <0.0001 |
| Host Species |
Rabbit |
| Immunogen |
3', 5'-cyclic AMP-8-KLH |
| Conjugate |
Unconjugated |
|
Working concentrations for specific applications should be determined by the investigator.The appropriate concentrations may be affected by secondary antibody affinity, antigen concentration, the sensitivity of the method of detection, temperature, the length of the incubations, and other factors.The suitability of this antibody for applications other than those listed below has not been determined.The following concentration ranges are recommended starting points for this product. |
| Application |
Recommended Usage |
| ELISA |
1:4,000-1:40,000 |
| Sensitivity |
Sensitivity is defined as the 90% intercept of a B/B0 standard curve.In competitive ELISA system the sensitivity has been found to be 1-50 fmol/well. |
|
| Form |
Liquid |
| Storage Buffer |
Antiserum containing 0.02% sodium azide |
| Concentration |
Not applicable |
| Storage Instructions |
The antibody is stable for 2-3 weeks if stored at 2-8°C.For long term storage, aliquot and store at -20°C or below.Avoid repeated freezing and thawing cycles. |
| Purification |
Antiserum |
| Isotype |
Rabbit IgG |
| Clonality |
Polyclonal |
| Clone ID |
Not applicable |
| Note |
Small volume of antibody will occasionally become entrapped in the cap of the vial during shipment and storage.Before use, centrifuge the vial briefly to bring down any liquid in the cap. |
| Target Background |
Adenosine 3', 5'-cyclic monophosphate (cyclic AMP;cAMP) is one of the most important intracellular secondary messengers for transduction events.cAMP is also involved in regulating neuronal, glandular, cardiovascular, immune, and other functions and actions.A number of hormones, such as ACTH, TSH, FSH, and LH, are known to activate cAMP through the action of the enzyme adenylate cyclase, which converts ATP to cAMP.There remains considerable interest in the measurement of intracellular cAMP in tissues and cell cultures, and this may help to provide an understanding of the physiology and pathology of many disease states.Due to the involvement of cAMP in amplifying the response of ligand binding, the second messenger cAMP has been largely employed to monitor the activation of GPCR to facilitate therapeutic drug discovery.GenScript Rabbit Anti-cAMP Polyclonal Antibody is developed in rabbit using 3', 5'-cyclic AMP-8-KLH as the immunogen. |
| Synonyms |
Rabbit Anti cAMP pAb; |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
