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Sequence and annotation of 42 cannabis genomes reveals extensive copy number variation in cannabinoid synthesis and pathogen resistance genes

biorxiv. 2020;

Kevin J. McKernan, Yvonne Helbert, Liam T. Kane, Heather Ebling, Lei Zhang, Biao Liu, Zachary Eaton, Stephen McLaughlin, Sarah Kingan, Primo Baybayan, Gregory Concepcion, Mark Jordan, Alberto Riva, William Barbazuk, Timothy Harkins

Products/Services Used	Details	Operation
Proteins, Expression, Isolation and Analysis	SDS-PAGE and B) Western blot analysis of CsTLP1 expression in E. coli. Lane M1: Protein marker (GenScript, Cat. No. M00516); Lane 1: Bovine serum albumin (2.00 μg); Lane 2: CsTLP1_2563 (reducing condition, 2.00 μg); Lane M2: Protein marker (GenScript, Cat. No. M00521); Lane 3: CsTLP1_2563 (reducing condition); primary antibody: mouse-anti-His mAb (GenScript, Cat. No. A00186).	Get A Quote

摘要

Cannabis is a diverse and polymorphic species. To better understand cannabinoid synthesis inheritance and its impact on pathogen resistance, we shotgun sequenced and assembled a Cannabis trio (sibling pair and their offspring) utilizing long read single molecule sequencing. This resulted in the most contiguous Cannabis sativa assemblies to date. These reference assemblies were further annotated with full-length male and female mRNA sequencing (Iso-Seq) to help inform isoform complexity, gene model predictions and identification of the Y chromosome. To further annotate the genetic diversity in the species, 40 male, female, and monoecious cannabis and hemp varietals were evaluated for copy number variation (C... More