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Escherichia coli surface display of single-chain antibody VRC01 against HIV-1 infection.

Virology.. 2014-12;  475C:179-186
LX Wang, M Mellon, D Bowder, M Quinn, D Shea, C Wood, SH Xiang. Nebraska Center for Virology, Lincoln, NE, USA.
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摘要

Human immunodeficiency virus type 1 (HIV-1) transmission and infection occur mainly via the mucosal surfaces. The commensal bacteria residing in these surfaces can potentially be employed as a vehicle for delivering inhibitors to prevent HIV-1 infection. In this study, we have employed a bacteria-based strategy to display a broadly neutralizing antibody VRC01, which could potentially be used to prevent HIV-1 infection. The VRC01 antibody mimics CD4-binding to gp120 and has broadly neutralization activities against HIV-1. We have designed a construct that can express the fusion peptide of the scFv-VRC01 antibody together with the autotransporter β-barrel domain of IgAP gene from Neisseria gonorrhoeae, which... More

关键词

Autotransporter; E. coli K12 UT5600; HIV-1; Surface display; scFv-VRC01; β-Barrel domain