| Species |
Mouse |
| Protein Construction |
FGF-21 (Ala29-Ser210)
Accession # Q9JJN1 |
|
| Purity |
> 97% as analyzed by SDS-PAGE
> 97% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 0.5 μg/ml,measured by a cell proliferation assay using NIH-3T3 cells in the presence of 1.25 µg/ml mouse Klotho and 10.0 µg/ml heparin, corresponding to a specific activity of > 2.0 × 103 units/mg. |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~19.9 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Lyophilized after extensive dialysis against PBS. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in ddH₂O or PBS up to 100 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Fibroblast growth factor-21 (FGF21) belongs to the large FGF family which has at least 23 members. Along with FGF-19/15 and FGF-23, FGF-21 is categorized as a member of the atypical FGF subfamily, as it must be complexed to the Klotho co-receptor in order to bind to the FGF receptors and activate the downstream signaling pathway. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. |
| Synonyms |
FGF21; fibroblast growth factor-21; FGFL |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
