|Synonyms||CXCL8, monocyte-derived neutrophil chemotactic factor (MDNCF), neutrophil activating factor (NAF), NAP-1|
|Description||Interleukin-8 is one of the first discovered chemokines, and belongs to the CXCL family, in which the first two conserved cysteines are separated by one residue. In vivo, IL-8 exists in two forms: 77 a.a. produced by endothelial cells, and the more active 72 a.a. produced by monocytes. The receptors of IL-8 are the seven-helical G-protein coupled receptors CXCR1 and CXCR2, exclusively expressed on neutrophils. The functions of IL-8 are to induce rapid changes in cellular shape, activate the integrins, and release the granule contents of neutrophils. Thus, IL-8 can enhance the antimicrobial actions of defense cells.
Recombinant human I Interleukin-8/CXCL8 (rhIL-8) produced in E. coli is a single non-glycosylated polypeptide chain containing 72 amino acids. A fully biologically active molecule, rhIL-8 has a molecular mass of 8.4 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 20 ng/mL, measured by the FLIPR assay using CHO cells transfected with human CXCR1, the receptor of human CXCL8, corresponding to a specific activity of > 5×104 units/mg.|
SAKELRCQCI KTYSKPFHPK FIKELRVIES GPHCANTEII
VKLSDGRELC LDPKENWVQR VVEKFLKRAE NS
|Measured Molecular Weight||8.4 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% as analyzed by SDS-PAGE and HPLC.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/mL.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Interleukin-8/CXCL8 (rhIL-8) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-8 remains stable up to 2 weeks at 4°C or up to 3 months at -20°C.|