|Synonyms||26 kDa protein, IFN-β2, B cell differentiation factor (BCDF), BSF-2, HPGF, HSF, MGI-2|
|Description||Interleukin-6 (IL-6, also known as IFN-β2) is a pleiotropic cytokine that plays important roles in acute phase reactions, inflammation, hematopoiesis, bone metabolism, and cancer progression. IL-6 is secreted by T cells and macrophages to stimulate immune response. IL-6 is responsible for stimulating acute phase protein synthesis, as well as the production of neutrophils in the bone marrow. It supports the growth of B cells and is antagonistic to regulatory T cells.
Recombinant human Interleukin-6 (rhIL-6) produced in E. coli is a single non-glycosylated polypeptide chain containing 185 amino acids. A fully biologically active molecule, rhIL-6 has a molecular mass of 20.9 kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 1 ng/ml, measured in a cell proliferation assay using TF-1 cells, corresponding to a specific activity of > 1 x 106 units/mg.|
MPVPPGEDSK DVAAPHRQPL TSSERIDKQI RYILDGISAL
RKETCNKSNM CESSKEALAE NNLNLPKMAE KDGCFQSGFN
EETCLVKIIT GLLEFEVYLE YLQNRFESSE EQARAVQMST
KVLIQFLQKK AKNLDAITTP DPTTNASLLT KLQAQNQWLQ
DMTTHLILRS FKEFLQSSLR ALRQM
|Measured Molecular Weight||20.9 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE analysis.|
|Formulation||Lyophilized after extensive dialysis against 1xPBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Interleukin-6 (rhIL-6) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-6 should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|