|Synonyms||Interferon alpha 2b, IFNA, INFA2, MGC125764, MGC125765|
|Description||Interferon-Alpha 2b (IFN-Alpha 2b) produced by leukocytes is a member of Interferon family. IFN-alpha is mainly involved in innate immune response against a broad range of viral infections. IFN-alpha 2 has three acid stable forms (a,b,c) signaling through IFNAR2. IFN-alpha 2b shares 99.4% aa sequence identity with both IFN-alpha 2a and 2c. IFN-alpha contains four highly conserved cysteine residues which form two disulfide bonds, one of which is necessary for biological activity.
Recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) produced in E.coli is a single non-glycosylated polypeptide chain containing 165 amino acids. A fully biologically active molecule, rhIFN-Alpha 2b has a molecular mass of 19.2kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.05 ng/ml, measured by a cytotoxicity assay using TF-1 Cells, corresponding to a specific activity of > 2.0× 107 units/mg.|
CDLPQTHSLG SRRTLMLLAQ MRRISLFSCL KDRHDFGFPQ
EEFGNQFQKA ETIPVLHEMI QQIFNLFSTK DSSAAWDETL
LDKFYTELYQ QLNDLEACVI QGVGVTETPL MKEDSILAVR
KYFQRITLYL KEKKYSPCAW EVVRAEIMRS FSLSTNLQES
|Measured Molecular Weight||19.2kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O at 100 μg/ml.|
|Endotoxin Level||< 0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Interferon-Alpha 2b (rhIFN-Alpha 2b) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIFN-Alpha 2b should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|