| Species |
Human |
| Protein Construction |
Expressed with additional C-terminal sequence (GGYGRKKRRQRRR).
NANOG-TAT [Met1-Val305 (Lys82Asn)]
Accession # Q9H9S0 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.2 EU/μg of protein by gel clotting method |
| Expression System |
E. coli |
| Apparent Molecular Weight |
~36.2 kDa, on SDS-PAGE under reducing conditions. |
| Formulation |
Supplied as a sterile filtered solution in 10 mM PB, 300 mM NaCl, pH 7.4. |
| Concentration |
Please refer to the COA for the specific lot. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -20°C or below and 1-2 weeks at 4°C. For long term storage, aliquot and store at -70°C or below. Avoid repeated freeze-thaw cycles. |
| Target Background |
NANOG is a transcription factor involved with self-renewal of inner cell mass and embryonic stem (ES) cells by functioning in concert with other factors such as POU5F1 (Oct-4) and SOX2. Nanog imposes pluripotency on ES cells and prevents their differentiation towards extraembryonic endoderm and trophectoderm lineages, and blocks bone morphogenetic protein-induced mesoderm differentiation of ES cells by physically interacting with SMAD1 and interfering with the recruitment of coactivators to the active SMAD transcriptional complexes. |
| Synonyms |
Homeobox protein NANOG; Homeobox transcription factor Nanog |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
