| Species |
Human |
| Protein Construction |
Expressed with an N-terminal Met.
NT-4 (Gly81-Ala210)
Accession # P34130 |
|
| Purity |
> 95% as analyzed by SDS-PAGE
> 95% as analyzed by HPLC |
| Endotoxin Level |
< 0.3 EU/μg of protein by gel clotting method |
| Biological Activity |
ED50 < 5.0 μg/ml, measured by a cell proliferation assay using C6 cells, corresponding to a specific activity of > 2.0 × 102 units/mg. |
| Expression System |
E. coli |
| Theoretical Molecular Weight |
28.1 kDa, a noncovalently linked homodimer of two 14.0 kDa polypeptide monomers. |
| Formulation |
Lyophilized after extensive dialysis against 50 mM acetic acid. |
| Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in 50 mM acetic acid or ddH₂O up to 50 μg/ml. |
| Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at lower than -70°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. For long term storage it is recommended that a carrier protein (example 0.1% BSA) be added. Avoid repeated freeze-thaw cycles. |
| Target Background |
Neurotrophin-4 (NT-4), also known as NT-5, is a neurotrophic factor structurally related to β-NGF, BDNF, and NT-3. Human NT-4 shares 48 - 52% aa sequence identity with human β-NGF, BDNF, and NT-3.Neurotrophins have six conserved cysteine residues that are involved in the formation of three disulfide bonds. NT-4 is expressed highest levels in prostate, lower levels in thymus, placenta, and skeletal muscle. NT-4 binds and induces receptor dimerization and activation of TrkB. NT-4 can signal through TrkB receptors and promotes the survival of peripheral sensory sympathetic neurons. |
| Synonyms |
Neurotrophin-4; NTF4; GLC10; GLC1O; NT-4; NT-4/5; NT-5; NT4; NT5; NTF5; neurotrophin 4 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.
