Species |
Rat |
Protein Construction |
CCL28 (Ser20-Arg135) Accession # Q91Y39 |
|
Purity |
> 96% as analyzed by SDS-PAGE > 96% as analyzed by HPLC |
Endotoxin Level |
< 1 EU/μg of protein by LAL method |
Biological Activity |
Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human lymphocytes is in a concentration range of 5.0-50.0 ng/ml. |
Expression System |
E. coli |
Theoretical Molecular Weight |
13.1 kDa |
Formulation |
Lyophilized from a 0.2 μm filtered solution in 20 mM PB, pH 7.4, 200 mM NaCl. |
Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/ml. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Target Background |
Mucosae-associated Epithelial Chemokine (MEC)/CCL28 (CC chemokine ligand 28) is a secreted CC chemokine expressed primarily by epithelial cells of the bronchioles, salivary gland, mammary gland and colon. MEC signals through the CCR10 receptor and chemoattracts resting CD4, CD8 T-cells and eosinophils. MEC contains six cysteines including the four highly conserved cysteine residues present in CC chemokines. |
Synonyms |
Chemokine (C-C motif) ligand 28; CCL-28; SCYA28; MGC71902; Small-inducible cytokine A28; Mucosae-associated epithelial chemokine; Protein CCK1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.