Species |
Rat |
Protein Construction |
BD-1 (Asp33-Ser69) Accession # O89117 |
|
Purity |
> 95% as analyzed by SDS-PAGE > 95% as analyzed by HPLC |
Endotoxin Level |
< 1 EU/μg of protein by LAL method |
Biological Activity |
Fully biologically active when compared to standard. The biological activity determined by a chemotaxis bioassay using CD34+ dendritic cells is in a concentration range of 100.0-1000.0 ng/ml. |
Expression System |
E. coli |
Theoretical Molecular Weight |
4.1 kDa |
Formulation |
Lyophilized from a 0.2 μm filtered solution in 20 mM PBS, 500 mM NaCl, pH 7.0. |
Reconstitution |
It is recommended that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute the lyophilized powder in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. |
Storage & Stability |
Upon receiving, this product remains stable for up to 6 months at -70°C or -20°C. Upon reconstitution, the product should be stable for up to 1 week at 4°C or up to 3 months at -20°C. Avoid repeated freeze-thaw cycles. |
Target Background |
Defensin are 3-4 kDa antimicrobial peptides of which three distinct families have been identified; α-defensin, β-defensins, and insect defensins. Rat β-Defensin 1, as a member of the β-defensin family, is a salt-sensitive antimicrobial peptide present in epithelia of the lung and urogenital tract. The predicted amino acid sequence shows the hallmark features of other known epithelial β-defensins, including the ordered array of six cysteine residues. |
Synonyms |
rBD-1; Defensin, beta 1; Defb1; Beta-defensin 1; Defensin beta 1 |
For laboratory research use only. Direct human use, including taking orally and injection and clinical use are forbidden.