|Synonyms||Catabolin, Lymphocyte-activating factor (LAF), Endogenous Pyrogen (EP), Leukocyte Endogenous Mediator (LEM), Mononuclear Cell Factor (MCF), IL1F2, IL-1 beta.|
|Description||Interleukin-1 beta (rhIL-1β) is a proinflammatory cytokine produced in a variety of cells including monocytes, tissue macrophages, keratinocytes and other epithelial cells. Both IL-1 alpha and IL-1 beta binds to the same receptor and has similar if not identical biological properties. These cytokines have a broad range of activities including, stimulation of thymocyte proliferation, by inducing IL-2 release, B-cell maturation and proliferation, mitogenic FGF-like activity and the ability to stimulate the release of prostaglandin and collagenase from synovial cells. However, whereas IL-1 beta is a secreted cytokine, IL-1 alpha is predominantly a cell-associated cytokine.
Recombinant human Interleukin-1 beta (rhIL-1β) produced in E. coli is a single non-glycosylated polypeptide chain containing 153 amino acids. A fully biologically active molecule, rhIL-1β has a molecular mass of 17.3kDa analyzed by reducing SDS-PAGE and is obtained by proprietary chromatographic techniques at GenScript.
|Biological Activity||ED50< 10 pg/ml, measured by the dose-dependent stimulation of mouse D10.G4.1 helper T cells, corresponding to a specific activity of 1.0×108 IU/mg.|
APVRSLNCTL RDSQQKSLVM SGPYELKALH LQGQDMEQQV
VFSMSFVQGE ESNDKIPVAL GLKEKNLYLS CVLKDDKPTL
QLESVDPKNY PKKKMEKRFV FNKIEINNKL EFESAQFPNW
YISTSQAENM PVFLGGTKGG QDITDFTMQF VSS
|Measured Molecular Weight||17.3 kDa, observed by reducing SDS-PAGE.|
|Purity||> 95% by SDS-PAGE and HPLC analyses.|
|Formulation||Lyophilized after extensive dialysis against PBS.|
|Reconstitution||Reconstituted in ddH2O or PBS at 100 μg/ml.|
|Endotoxin Level||<0.2 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Interleukin-1 beta (rhIL-1β) remains stable up to 6 months at lower than -70°C from date of receipt. Upon reconstitution, rhIL-1β should be stable up to 2 weeks at 4°C or up to 3 months at -20°C.|