|Synonyms||Granulocyte Macrophage Colony Stimulating Factor, CSF-2, MGI-1GM, GM-CSF, Pluripoietin-alpha, Molgramostin, Sargramostim, MGC131935, MGC138897|
|Description||Human Granulocyte Macrophage Colony Stimulating Factor (hGM-CSF), a hematopoietic growth factor, is mainly involved in granulopoiesis and monocytopoiesis. It is produced by T-cells and macrophages in response to antigens, and by endothelial cells and fibroblasts following induction of variouscytokines . A monomeric protein of 127 amino acidswith six glycosylation sites and two intra disulfide bonds [2,3], glycosylated and non-glycosylated hGM-CSFs show similar biological activities .Other than its connection to the growth and development of granulocytes and macrophages, it is also indispensable for the proliferation of erythroid and megakaryocytic cells .
Recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) produced in E. coli is a non-glycosylated polypeptide. A fully biologically active molecule, rhGM-CSF has a molecular mass of 14 kDa analyzed by SDS-PAGE and is obtained by proprietary refolding and chromatographic techniques at GenScript.
|Biological Activity||ED50 < 0.5 ng/ml, measured by proliferation assay of TF-1 cells, corresponding to a specific activity of >2.0 x 106units/mg|
MAPARSPSPS TQPWEHVNAI QEARRLLNLS RDTAAEMNET
VEVISEMFDL QEPTCLQTRL ELYKQGLRGS LTKLKGPLTM
MASHYKQHCP PTPETSCATQ IITFESFKEN LKDFLLVIPF
|Measured Molecular Weight||14 kDa, observed by SDS-PAGE.|
|Purity||>95 % as analyzed by SDS-PAGE.|
|Formulation||Lyophilized after extensive dialysis against 10 mM PB, pH7.0.|
|Reconstitution||Reconstituted in ddH2O at 100μg/ml.|
|Endotoxin Level||<1 EU/μg, determined by LAL method.|
|Storage||Lyophilized recombinant human Granulocyte Macrophage Colony Stimulating Factor (rhGM-CSF) remains stable up to 12 months at lower than -70°C from date of receipt. Upon reconstitution, rhGM-CSF should be stable up to 4 weeks at 4°C or up to 6 months at -20°C.|