IL-10, Mouse
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Interleukin-10 (IL-10) is produced by murine T-cells following their stimulation by lectins. The main source for B-cell-derived Interleukin-10 (IL-10) in mice is Ly-1 B-cells that express CD5 and CD11. Murine keratinocytes also produce Interleukin-10 (IL-10). In human Interleukin-10 (IL-10) is produced by activated CD8(+) peripheral blood T-cells, by T-helper CD4(+) T-cell clones (resembling Th0, Th1, and Th2) after both antigen-specific and polyclonal activation, by B-cell lymphomas, and by mococytes following cell activation by bacterial lipopolysaccharides and mast cells. B-cells lines derived from patients with acquired immunodeficiency syndrome and Burkitt's lymphoma constitutively secerete large quantities of Interleukin-10 (IL-10) into the conditioned medium. The synthesis of Interleukin-10 (IL-10) by monocytes is inhibited by IL-4 and IL-10. Recombinant mouse Interleukin-10 (IL-10) produced in E. coli is a single, non-glycosylated polypeptide chain containing 161 amino acids and having a molecular mass of 18,885 Da.
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