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Description: |
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| The Smart His-tagged Potein Standard is a lyophilized mixture of six highly purified recombinant proteins ranging in size from 15 to 115 kDa containing a terminal 6xHis tag. It yields six clear bands of similar intensity on western blots, thus can be used for precise MW determination of proteins on western blot membranes. | |||||||||||||||
Concentration: |
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| 7.5 μg of each protein in 60 mM Tris-HCl (pH6.8 at 25掳C), 0.4% (w/v) SDS, 10 mM DTT, 0.01% (w/v) bromophenol blue and 2% sucrose. | |||||||||||||||
Volume: |
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| Add 250 μl of 1x reconstitution buffer, 2.5 μl of the solution is recommended for each lane of a mini-gel. | |||||||||||||||
Storage: |
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| Store at -20掳C or below. After reconstitution, aliquot and store at -20掳C or below. | |||||||||||||||
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MSDS: 20090520063611 (PDF) |
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PRODUCTINFO: 20070402024608 (PDF) |
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EXAMPLE:
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