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Description: |
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| Protein L is a cell surface protein from Peptostreptoccocus magnus that binds to the variable light chains (kappa chain) of immunoglobulins without interfering with antigen binding. In contrast to IgG-binding proteins, such as protein A and protein G, protein L can be used for the detection and purification of mammalian kappa light chain antibodies of all classes. Biotin-Protein L, which is produced by covalently linking biotin to protein L, can be used in immunodetections, such as immunoblotting (western or dot blot), ELISA, and immunohistochemistry. | |||||||||||||||
Concentration: |
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| 1 mg/ml in PBS (pH 7.4) containing 10 mg/ml BSA and 0.02% sodium azide. | |||||||||||||||
Storage: |
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| Store at 4℃ for short periods of time, one month or shorter. Aliquot and store at -20℃ for longer periods. Avoid repeated freeze/thaw cycles. Prolonged storage may result in slight precipitation, so clarify the solution by centrifugation before use. | |||||||||||||||
Application: |
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| Working concentrations for specific applications should be determined by titration assay. The chart below may serve as a useful guideline, but primary antibody, temperature, incubation time, the amount of antigen, and the sensitivity of the detection methods can all affect the ideal working concentration. Dot blot: 1:2,000-1:20,000 Western blot: 1:2,000-1:20,000 ELISA: 1:20,000-1:100,000 Other: user-optimized |
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PRODUCTINFO: 20070208033901 (PDF) |
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