|
|||||||||||||||||
Description: |
|||||||||||||||||
| Protein G, a cell surface protein of Group G streptococci, is a Type III Fc receptor that binds to the Fc region of IgG by a non-immune mechanism similar to that of protein A. It contains 3 IgG binding domains and is engineered to delete albumin-binding region. HRP has been covalently coupled to Protein G to produce the HRP-Protein G, which can be used in detection systems such as immunoblotting, ELISA, immunoperoxidase electron microscopy and immunohistochemistry. | |||||||||||||||||
Concentration: |
|||||||||||||||||
| 2 mg/ml in PBS, pH 7.4, containing 10 mg/ml BSA and 0.01% Thimerosal. | |||||||||||||||||
Storage: |
|||||||||||||||||
| Store at 4℃ for short term. Aliquot and store at -20℃ or -80℃. Avoid repeated freeze/thaw cycles. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. | |||||||||||||||||
Application: |
|||||||||||||||||
| Suitable for immunoblotting (western or dot blot), ELISA, immunoperoxidase electron microscopy and immunohistochemistry as well as other peroxidase-antibody based enzymatic assays requiring lot-to-lot consistency. Working concentrations for specific applications should be determined by the investigator. Appropriate concentrations will be affected by several factors, including primary antibody affinity, antigen concentration, sensitivity of detection method, temperature, and length of incubations, etc. The suitability of this antibody for applications other than those listed below has not been determined. The following concentration ranges are recommended starting points for this product. Dot blot: 1:2,000~1:10,000 Western blot: 1:2,000~1:10,000 ELISA: 1:10,000~1:50,000 Immunohistology: 1:1,000~1:10,000 |
|||||||||||||||||
|
MSDS: 20080916031204 (PDF) |
|||||||||||||||||
|
PRODUCTINFO: 20060920233835 (PDF) |
|||||||||||||||||
