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Description: pRNA-H1.3/Neo/siFLuc is a Genscript siRNA expression vector. It is designed for mammalian transfection. It uses an enhanced H1 promoter (enhanced by a CMV enhancer just before H1 promoter) for siRNA expression. This vector contains siRNA construct for Firefly luciferase, and can be used as a positive control. It can also be used as a siRNA vector using BamH I and Hind III for siRNA insertion. |
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| Download Protocol:tm0169 | |||||||||||||||
| Forward Sequencing Primer: DA0024:pRNA-H1.3 Forward (GACGTCAATGGGAGTTTGTT) |
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| Reverse Sequencing Primer: DA0012:pRNA Reverse (TAGAAGGCACAGTCGAGG) |
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| Detailed Map: |
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| CMV IE:4559-5042 | |||||||||||||||
| siFluc:5148-5205 | |||||||||||||||
| Polylinker:5142-5211 | |||||||||||||||
| H1 Promoter:5049-5143 | |||||||||||||||
| SV40:647-992 | |||||||||||||||
| Neomycin:1033-1827 | |||||||||||||||
| pUC ori:2541-3181 | |||||||||||||||
| Ampicillin:3329-4189 | |||||||||||||||
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DATASHEET: 20051024030347 (PDF) |
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