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| The human cytomegalovirus (CMV) promoter is one of the strongest promoters described. Based on the RNA polymerase II system, the CMV promoter drives higher-level constitutive expression of genes in a greater variety of mammalian cell lines than RNA polymerase III-based promoters, such as U6 and H1. A CMV promoter drives the expression of siRNA, and an SV40 promoter drives the expression of the resistance gene. siRNA cassettes can be easily inserted into the vectors between BamH I and Afl II sites. The siRNA sequence can be confirmed by DNA sequencing using reverse sequencing primer DA0012 (5?TAGAAGGCACAGTCGAGG-3?. This vector also carries cGFP (coral GFP) for convenient tracking of transfection efficiency. | |||||||||||||||
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| Download Protocol:tm0172 | |||||||||||||||
| Forward Sequencing Primer: DA0023:pRNA-CMV Forward (GTACGGTGGGAGGTCTATAT) |
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| Reverse Sequencing Primer: DA0012:pRNA Reverse (TAGAAGGCACAGTCGAGG) |
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| Detailed Map: |
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| Puromycin:2911-3510 | |||||||||||||||
| Polylinker:584-651 | |||||||||||||||
| CMV Promoter:1-583 | |||||||||||||||
| cGFP:1598-2317 | |||||||||||||||
| SV40:1267-1598 | |||||||||||||||
| pUC ori:4210-4850 | |||||||||||||||
| Ampicillin:4998-5858 | |||||||||||||||
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PRODUCTINFO: 20051024022606 (PDF) |
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PROTOCOL: 20050718094354 (PDF) |
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| Cat. No. | Name | Size | Price(￥) |
|---|---|---|---|
| A00185 |
antibody : cGFP-tag Antibody, mAb, Mouse | 40 | 550.00 |
| 200 |