pRNA-H1.1/Retro

Cat. No. Name Size Price(¥)
SD1241 
pRNA-H1.1/Retro  10 ug  2475.00 
FullName pRNA-H1.1/Retro


Description: pRNA-H1.1/Retro siRNA expression vector is compatible with Clontech Retro-X Expression System. An H1 promoter drives siRNA expression. the siRNA insert cloned between the Mlu and Xho sites. The vector contains a hygromycin resistance gene under the control of cytomegalovirus (CMV) promoter* for establishing stable cell line. The vector uses CMV enhancer/promoter joined MSV 5???TR (CMV/MSV 5'LTR). It uses MSV 3'LTR for viral transcription and packaging.

Download Protocol:TM0187
Forward Sequencing Primer:
DA0025:SD1241 Forward (TGTAGGTTTGGCAAGCTAGC)
Reverse Sequencing Primer:
DA0013:pRNA-H1 Forward (TAATACGACTCACTATAGGG)
Detailed Map:
5' LTR:1-727
3' LTR:4768-5063
psi+:757-1566
ColE1 ori:6650-5764
SV40 ori \& promoter:5320-5664
Polylinker:4596-4619
H1 Promoter:4719-4620
CMV Promoter:1604-2132
Hygromycin:2901-3921
Ampicillin:7570-6710
Publications used this GenScript Product:
  • Qing Shao, Hongling Wang, Elizabeth McLachlan, Gregory I.L. Veitch, and Dale W. Laird. Down-regulation of Cx43 by Retroviral Delivery of Small Interfering RNA Promotes an Aggressive Breast Cancer Cell Phenotype. Cancer. Res. Apr 2005; 65 (7) :2705-2711


  • PRODUCTINFO:
    20051024014300 (PDF)
    PROTOCOL:
    20050708115745 (PDF)