ONE-HOUR WesternTM Complete Kit Using TMB (Goat)
Order:
Cat. No. Name Size Price(￥)
L00228T 
 ONE-HOUR WesternTM Complete Kit Using TMB (Goat)  1 kit  2805.00 
FullName ONE-HOUR WesternTM Complete Kit Using TMB (Goat)
Components:

Components L00204C L00204T L00205T L00205C L00228 L00228T 
High-Sensitivity X-Ray Film
(5 x 7 inches)  		20 sheets X X 20 sheets 20 sheets X 
LumiSensorTM Chemiluminescent HRP Substrate 2 x 15 ml X X 2 x 15 ml 2 x 15 ml X 
Pretreat Solution A 100 ml 100 ml 100 ml 100 ml 100 ml 100 ml 
Pretreat Solution B 100 ml 100 ml 100 ml 100 ml 100 ml 100 ml 
Protocol 1 1 1 1 1 1 
WB-1 Solution 1 ml 1 ml 1 ml 1 ml 1 ml 1 ml 
WB-2 Solution 100 ml 100 ml 100 ml 100 ml 100 ml 100 ml 
WestClearTM Nitrocellulose Membrane
(0.2 .m, 7.5 x 8 cm) 		10 sheets 10 sheets 10 sheets 10 sheets 10 sheets 10 sheets 
10X Wash Solution 125 ml 125 ml 125 ml 125 ml 125 ml 125 ml 
ChromoSensorTM One Solution TMB Substrate X 60 ml 60 ml X X 60 ml 



Kit Description

GenScript's ONE-HOUR WesternTM Kit yields journal-quality Western or Dot blot results in about one hour. Using GenScript's breakthrough immunodetection technology (patent pending), the kit replaces the classical three-step Western process, which can take nearly five hours. Transfer the proteins from gel to membrane and incubate it in the pretreat solution for five minutes. Then incubate in WB-2 solution with the mixture of primary antibody and WB-1 solution for 40 minutes. Then wash three times for five or ten minutes each. The membrane can then be developed with the HRP substrate. The ONE-HOUR WesternTM procedure is contrasted with a classical three-step Western at right.

The ONE-HOUR WesternTM Kit is designed to produce high signal with low background for quick and clear Western analysis of proteins.

Except for basic kits, all other kits contain all the necessary reagents, buffers and materials such as membrane and X-ray films for performing a Western blot, and no additional secondary antibody is needed.

The ONE-HOUR WesternTM Kit saves precious research time. For added cost-efficiency, we recommend our complete kits, which contain all the reagents, membrane, HRP substrate, and X-ray film necessary for a perfect blot. However, the kit components are also sold separately.

Key Features

  • Easy to perform: This kit has fewer and simpler steps than other Western kits.
  • Low background: The kit contains WestClearTM Nitrocellulose Membrane and LumiSensorTM series of Chemiluminescent HRP Substrate Kits, and X-ray film, optimized for low background.
  • Reproducible results: The kit produces highly reproducible results.
  • No additional secondary antibody is needed.

Examples:

Comparison of the two ONE-HOUR WesternTM Blot Kits of different sensitivities using monoclonal antibodies:
Two similar blots were processed with the same procedures using different ONE-HOUR WesternTM Kits: Standard (L00205C) and Advanced (L00242). 10 μg and 2.5 μg of THETM Anti-GST Monoclonal Antibody (Mouse) (GenScript, A00865), respectively, were used with these two kits to detect GST protein.
The results are shown in Figure 1.

Figure 1. Western blots for the detection of GST protein by ONE-HOUR WesternTM using different ONE-HOUR WesternTM Kits: Standard (L00205C) and Advanced (L00242). 33.0, 11.0, 3.60, 1.20, 0.41, 0.13 and 0.045 ng of GST protein were loaded into Lanes 1, 2, 3, 4, 5, 6, and 7 respectively.

Comparison of the two ONE-HOUR WesternTM Blot Kits of different sensitivities using polyclonal antibodies:
Two similar blots were processed with the same procedures using different ONE-HOUR WesternTM Kits: Standard (L00204C) and Advanced (L00241). 10 μg and 2.5 μg of Rabbit Anti-GST-tag Polyclonal Antibody (GenScript, A00097), respectively, were used with the two kits to detect GST protein.
The results are shown in Figure 2.

Figure 2. Western blots for the detection of GST protein by ONE-HOUR WesternTM using different ONE-HOUR WesternTM Kits: Standard (L00204C) and Advanced (L00241). 25.0, 12.5, 6.25, 3.10, 1.50, and 0.78 ng of GST protein were loaded into Lanes 1, 2, 3, 4, 5, and 6 respectively.
MSDS:
20060818160706 (PDF)