LumiSensorTM HRP Substrate Kit
Order:
Cat. No. Name Size Price(￥)
L00221V60 
 LumiSensorTM HRP Substrate Kit  1 Kit (2 X 30ml)  605.00 
FullName LumiSensorTM HRP Substrate Kit



Description


LumiSensorTM Chemiluminescent HRP Substrate Kit renders horseradish peroxidase (HRP) detectable on immunoblots such as Western and Dot blots. The reagents are optimized for high sensitivity, high intensity, long duration, and low background.

Key Features :
  • Easy to perform: The procedure is quick and simple, with only a three-minute incubation period.
  • High sensitivity and low background: The kit is optimized to yield clear results each time.
  • Reproducible results: The kit produces highly reproducible results.
Examples:

Shown below is a Dot blot detection of IL-8 protein.
Figure 1. A Dot blot for IL-8 protein using the One-Step WesternTM Blot Kit (GenScript, L00205) and Mouse IL-8 Antibody (Endogen, M801). 120 ng, 40.0 ng, 13.3 ng, 7.4 ng, 4.4 ng, and 1.5 ng of IL-8 protein were spotted on the membrane. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit.
Examples of using One-Step<sup>TM</sup> GST Western Blot Kit
Shown below is a Western blot detection of housekeeping protein α-Tubulin using polyclonal antibody.
A Western blot for α-Tubulin using the One-Step WesternTM Blot Kit (GenScript, L00204) and polyclonal α-Tubulin antibody (abcam, ab4074). 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit.
Examples of using One-step Western Blot Kit
Shown below is a Western blot detection of housekeeping protein GAPDH using monoclonal antibody.
A Western blot for GAPDH using the One-Step WesternTM Blot Kit (GenScript, L00205) and Mouse GAPDH Antibody (abcam, ab8245). 10 μg, 5.0 μg, 2.5 μg, 1.25 μg, 0.62 μg, 0.31 μg, 0.16 μg, and 0.08 μg of Hela cell lysate (BD Biosciences, 611449) were loaded into Lane 1, Lane 2, Lane 3, Lane 4, Lane 5, Lane 6, Lane 7, and Lane 8, respectively. The blot was developed with the LumiSensorTM Chemiluminescent HRP Substrate Kit.
Examples of using One-step Western Blot Kit
MSDS:
20090924044446 (PDF)